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Efficientlycloneanysizeinsertupto200kb.CreatelibrariesfromA/T-orG/C-richgenomes.Clonegeneclustersoroperons.HighinsertstABIlity.InducIBLecopynumber.Lucigen'sCopyRight®v2.0BACcloningkitswithpSMART®BAC(Figure1)orpEZBAC(Figure2)vectorandBAC-OptimizedReplicator™v2.0ElectrocompetentCellsresultinunprecedentedaccuracy,reliability,andefficiencyinBACcloningandlibraryconstruction.ThesevectorsincorporatetheCloneSmart®transcription-freetechnology,whichminimizescloningbiasandenablescloningofotherwisetoxicgenes,alongwithinduciblecopynumberamplification(20-50X)forhighDNAyieldsandalacZ-sacBstufferfragmentthateliminatesuncutvectorfromrecombinants,resultinginzerobackground. UnlikeotherBACcloningsystems,Lucigen'sCloneSmarttechnologyassuresthateven"unclonable"insertsarestablymaintained.Sequencesarenotlostorrearrangedinthecloningprocess. CopyRightKitsoffersuperiorperformanceforBACcloningatamuchbetterpricethanotherkits. Figure3showsanexampleofaRandomShearBAClibrarygeneratedwithpSMARTBAC.Figure1.CopyRightvectorpSMARTBAC. ori2,repE,IncC-single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C-partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lamBDapackagingsignal;T-CloneSmarttranscriptionterminators;sacB-sucrasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.Figure2.CopyRightvectorpEZBAC.ori2,repE,IncC,single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C,partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lambdapackagingsignal;lacZ-alphapeptideofthebetagalactosidasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated. Figure3.ArabidopsisRandomShearBACLibraryinthepSMARTBACvector.7,680cloneswereobtained,withanaverageinsertsizeof105kband~5Xgenomecoverage.ConvenientSuccessEfficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,highlyefficientelectrocompetentcells,detailedinstructions,andatrouble-shootingguide.ORDERINFORMATIONEachCopyRight®v2.0BACCloningKitcontains:pre-cutvector(pSMARTBACwithachoiceofBamHIorHindIIIsites),CopyRightDNALigase,CopyRight5XLigationBuffer,PositiveControlInsertDNA,SequencingPrimers,BAC-OptimizedReplicatorv2.0ElectrocompetentCellsinDUOpackaging(2transformationspertube),PositiveControlInsertDNA,RecoveryMedium,ArabinoseInductionSolution,andcompleteprotocols.EachCopyRightpEZBACCloningKitcontains:pre-cutCopyRightvector(pEZBACBlunt),CloneSmartDNALigase,CloneDirect™10XLigationBuffer(includesATP),PositiveControlInsertDNA,ReplicatorInductionSolution,SequencingPrimers,andcompleteprotocols.KitswithcellsalsoincludeBAC-OptimizedReplicatorv2.0ElectrocompetentCells(>1×1010 cfu/µgDNA)packagedintwotransformationspertube(DUOs),PositiveControlPlasmid,andRecoveryMedium.Pleasenotethatthefollowingvectorshavebeendiscontinued:pSMARTBACEcoRI(catalognumbers42031-1,42031-2)andpEZBACBamHI(catalognumbers42007-1,42007-2and42012-1).”
美国Lucigen公司,自1998年成立至今,一直致力于生命科学领域相关科研产品的研究与开发,在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。
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