首页 > Lucigen > Lucigen/CopyRight v2.0 Fosmid Cloning Kit/42027-1/5 rxns

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Lucigen/CopyRight v2.0 Fosmid Cloning Kit/42027-1/5 rxns

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Efficientlycloneanyinsertof35-45kb.GetmorerecombinantsfromlessDNA.HighinsertstABIlity.InducIBLecopynumber.ImprovedFosmidCloningLucigen’sCopyRight®v2.0FosmidCloningKitwiththepSMART®FOSvectorandReplicator™FOSstraincontainsthemostadvancedfosmidcloningdeveloped.TheCopyRightv2.0FosmidKityieldsmanymorerecombinantsfromlimitedstartingDNA(Figure1). ThevectorincorporatesCopyRighttranscriptionterminatorstostabilizeinserts,whichresultsincompletefosmidlibrarieswithoutmissingclones.False-negativesandfalse-positivesaredramaticallyreduced,andempty-vectorbackgroundisessentiallyeliminated(Figure2).ControllablegeneticelementsinthepSMARTFOSvector(Figure3)andReplicatorFOScellsallowamplificationofvectorcopynumberforhighplasmidyields(Figure4).Figure1.HigherrecombinantyieldswithalimitingamountofDNA. Lucigen’sCopyRightFosmidkitcomparedtoEpicentre’sCopyControlkit.Figure2.ZerobackgroundwiththepSMARTFOSvector(left)comparedtotheCopyControl™pCC1FOS™vector(right)(Epicentre).Figure3.CopyRightv2.0pSMARTFOSvector.sacB,sucrasegene;lacZ,lacZalphapeptidegene;Cm^r-chloramphenicolresistancegene;cosN-lamBDapackagingsignal;parA,B,C-partitiongenes;ori2,repE[notshownindrawing],IncC-single-copyoriginofreplication;oriV-inducibleoriginofreplication.Approximatepositionsofsequencingprimersandtranscriptionterminators(T)areindicated.ThelacZ/sacBstufferregioniscompletelyremovedduringvectorprocessing.Figure4.CopynumberamplificationoffosmidclonesfromtheCopyRightv2.0FosmidKit(Replicatorcells)wasmoreconsistentandrobustthantheCopyControlFosmidkit(EPI300cells).ConvenientSuccessEfficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCtestingexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,optimizedcells,detailedinstructions,andtrouble-shootingguide.ORDERINFORMATIONEachCopyRightv2.0FosmidCloningKitcontains: pre-cut,dephosphorylatedpSMARTFOSbluntvector,CloneSmartDNALigase,CloneDirect™10XLigationBuffer(includesATP),DNATerminatorEndRepairEnzymeMixandEndRepairBuffer,ReplicatorFOSstrain(glycerolstock),20%MaltoseSolution,1MMgSO4,SMBuffer,ArabinoseInductionSolution,SequencingPrimers,andcompleteprotocols.The20-reactionKitisprovidedastwo10-reactionKits.

美国Lucigen公司，自1998年成立至今，一直致力于生命科学领域相关科研产品的研究与开发，在分子生物学领域处于领导性地位。Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、ClonePlex™ AK文库构建试剂盒、DNA Terminator ®末端修复试剂盒、EconoTaq® DNA聚合酶及E.cloni感受态细胞等。Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。