OverviewProduct nameHuman Alpha-synuclein ELISA KitSee all Alpha-synuclein kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Serum82.5%Inter-assaySamplenMeanSDCV%Serum38.9%Sample typeSerum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma, Tissue LysateAssay typeSandwich (quantitative)Sensitivity 504 pg/mlRange615 pg/ml -7000 pg/mlRecoverySample specific recoverySample typeAverage %RangeSerum117116%- 118%Cell culture media10390%- 114%Hep Plasma10693%- 115%EDTA Plasma118105%- 129%Cit plasma113113%- 113%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:HumanProduct overviewHuman Alpha-synuclein ELISA Kit (ab260052) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Alpha-synuclein protein in cell culture media, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Alpha-synuclein with 504 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.ASSAY SPECIFICITYThis kit recognizes both native and recombinant human Alpha-synuclein protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.Urine, saliva, and cerebrospinal fluid samples have not been tested with this kit.CROSS REACTIVITYRecombinant Tau 441, UCHL-1, beta-tubulin, and Gamma-synuclein were prepared at 7,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.INTERFERENCERecombinant Tau 441, UCHL-1, and beta tubulin were prepared at 4 ng/mL and tested for interference. No interference was observed.SPECIES REACTIVITYThis kit recognizes human Alpha-synuclein protein.Other species reactivity was determined by measuring 50% serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.This kit was found to react with rhesus macaque serum.Reactivity < 3%="" was="" determined="" for="" the="" following="">MouseRatCowOther species reactivity not determined.NotesAlpha-synuclein is a 140- amino acid protein encoded by the SNCA gene, primarily found in neural tissue. It is a member of the synuclein family of proteins, including β-Synuclein and γ-Synuclein. Alpha-synuclein has been found concentrated in the presynaptic nerve terminals of neurons and has been found in the nucleus of neurons. Alpha-synuclein is a major component of Lewy Bodies. This protein appears to play a role in dopamine metabolism and vesicle trafficking.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.PlatformPre-coated microplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Human Alpha-synuclein Capture Antibody1 x 600µl10X Human Alpha-synuclein Detector Antibody1 x 600µl10X Wash Buffer PT (ab206977)1 x 20ml5X Cell Extraction Buffer PTR (ab193970)1 x 10mlAntibody DiluentCPI - HAMA Blocker (ab193969)1 x 6mlHuman Alpha-synuclein Lyophilized Recombinant Protein (ab51189)2 vialsPlate Seals1 unitSample Diluent NS (ab193972)1 x 50mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasNeuroscienceNeurology processNeurodegenerative diseaseParkinson"s diseaseSynucleinNeuroscienceNeurology processNeurodegenerative diseaseAlzheimer"s diseaseTangles & TauFunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.Parkinson disease 1Parkinson disease 4Dementia Lewy bodySequence similaritiesBelongs to the synuclein family.DomainThe "non A-beta component of Alzheimer disease amyloid plaque" domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.Post-translationalmodificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.Ubiquitinated. The predominant conjugate is the diubiquitinated form.Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.Target information above from: UniProt accessionP37840The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesAlpha synuclein Alpha-synucleinAlpha-synuclein, isoform NACP140 alphaSYNMGC105443MGC110988MGC127560MGC64356NACPNon A beta component of AD amyloid Non A4 component of amyloidNon A4 component of amyloid precursor Non-A beta component of AD amyloidNon-A-beta component of alzheimers disease amyloid , precursor ofNon-A4 component of amyloid precursorNon-A4 component of amyloid, precursor ofOTTHUMP00000218549OTTHUMP00000218551OTTHUMP00000218552OTTHUMP00000218553OTTHUMP00000218554PARK 1PARK 4PARK1PARK4Parkinson disease (autosomal dominant, Lewy body) 4Parkinson disease familial 1SNCASnca synucleinSnca synuclein, alpha (non A4 component of amyloid precursor)SYNSynuclein alpha Synuclein alpha 140Synuclein, alpha (non A4 component of amyloid precursor)SYUA_HUMANsee allDatabase linksEntrez Gene:6622 HumanOmim:163890 HumanSwissProt:P37840 HumanUnigene:21374 Human