DescriptionProduct nameRecombinant human EGF protein (Animal Free)See all EGF proteins and peptidesBiological activityThe ED50,as determined by a cell proliferation assay using balb/c 3T3 cells, is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.Purity>= 98% SDS-PAGE.Sterile filtered.Greater than 98% pure by HPLC analyses.Endotoxin levelExpression systemEscherichia coliAccessionQ6QBS2Protein lengthFull length proteinAnimal freeYesNatureRecombinantSpeciesHumanSequenceNSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELRPredicted molecular weight6 kDaAmino acids971 to 1023Additional sequence informationab9697 is a globular protein containing 53 amino acid residues, including 2 intramolecular disulfide bonds.Associated productsRelated ProductsAnti-EGF antibody (ab9695)SpecificationsOur Abpromise guarantee covers the use ofab9697in the following tested applications.The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.ApplicationsWestern blotCellular ActivationSDS-PAGEHPLCFormLyophilizedAdditional notesManufactured using all non-animal reagents.Concentration information loading...Preparation and StorageStability and StorageShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.This product is an active protein and may elicit a biological response in vivo, handle with caution.ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.General InfoAlternative namesBeta urogastronebeta-urogastroneEGFEGF_HUMANEpidermal growth factorHOMG4OTTHUMP00000219721OTTHUMP00000219722Pro epidermal growth factorURGUrogastronesee allFunctionEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).Tissue specificityExpressed in kidney, salivary gland, cerebrum and prostate.Involvement in diseaseHypomagnesemia 4Sequence similaritiesContains 9 EGF-like domains.Contains 9 LDL-receptor class B repeats.Post-translationalmodificationsO-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).Cellular localizationMembrane.Target information above from: UniProt accessionP01133The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProt