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Abcam/Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets) (ab133993)/1 x 4 Membranes/Membrane, 22 Targets) (ab133993
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OverviewProduct nameCytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets)See all Cytokine Antibody Array antibody arraysSample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture mediaAssay typeSemi-quantitativeSpecies reactivityReacts with:MouseProduct overviewab133993 is for simultaneous detection of 22 Mouse Cytokines. Suitable for all sample types.Targets: GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, RANTES, SCF, sTNF RI, TNF-alpha, Thrombopoietin, VEGFCytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP.The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.Learn more about membrane antibody arraysNotesIf you are interested in this cytokine array, a tableof mouse cytokine membrane arrays and the analytes they measure is available here.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Tested applicationsSuitable for:Multiplex Protein Detectionmore detailsPropertiesStorage instructionsStore at -20°C. Please refer to protocols.Components1 x 4 Membranes1 x 8 Membranes1 x 2 Membranes1,000X HRP-Conjugated Streptavidin1 x 50µl1 x 50µl1 x 50µl1X Blocking Buffer1 x 25ml2 x 25ml1 x 25ml20X Wash Buffer I1 x 10ml1 x 20ml1 x 10ml20X Wash Buffer II1 x 10ml1 x 20ml1 x 10ml2X Cell Lysis Buffer1 x 10ml1 x 16ml1 x 10ml8-Well Incubation Tray (with Lid)1 unit1 unit1 unitBiotin-Conjugated Anti-Cytokines2 vials4 vials1 vialCytokine Antibody Array Membranes4 units2 x 4 units2 unitsDetection Buffer C1 x 1.5ml1 x 2.5ml1 x 1.5mlDetection Buffer D1 x 1.5ml1 x 2.5ml1 x 1.5mlResearch areasImmunologyInnate ImmunityCytokinesInterleukinsImmunologyInnate ImmunityCytokinesInterferonsAntibody ArraysMembrane antibody arraysCytokine antibody arrays
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