OverviewProduct nameFura-2 AM, Ca2+ selective fluorescent indicatorDescriptionHigh affinity Ca2+ selective fluorescent indicatorAlternative namesFura 2 acetoxymethyl esterBiological descriptionHigh affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.Purity>95%General notes1 unit = 50μg1 mg - 1 x 1 mg vial20 units - 20 x 50 µg vials10 units - 10 x 50 µg vials50 µg - 1 x 50 µg vialRelated protocols:Fura-2 AM imaging protocolCalcium imaging using Fura-2 AM in motor neuronsMeasurement of free cytosolic calcium concentration in single CHO-K1 cellsCAS Number108964-32-5 Chemical structurePropertiesChemical name2-[6-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl esterMolecular weight1001.86Molecular formulaC44H47N3O24PubChem identifier3364574Storage instructionsShipped at Room Temperature. Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.Solubility overviewSoluble in DMSO to 10 mMHandlingWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.SMILESCC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)CSourceSyntheticResearch areasBiochemicalsChemical TypeBiochemicalsBiochemicalsChemical TypeFluorescent toolsBiochemicalsPharmacologySignalingCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaAlzheimer"s DiseaseSignalingCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaDiabetesCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaDiabetesSignalingCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaHeart diseaseCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaHypertensionCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaHypertensionSignalingCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaObesityCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaObesitySignalingCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaPain & inflammationCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaRespiratory diseaseCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaStrokeCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsResearch AreaStrokeSignalingCa2+ signalingIonophores, Indicators & ChelatorsKits/ Lysates/ OtherTools and ReagentsFluorescent dyes and reagentsBiochemicalsResearch AreaCancerCa2+ signalingIonophores, Indicators & ChelatorsBiochemicalsChemical TypeDyes, stains and fluorescent tools