OverviewProduct nameHuman Galectin-3 ELISA Kit (LGALS3)See all Galectin 3 kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Serum83.4%Inter-assaySamplenMeanSDCV%Serum31.6%Sample typeCell culture supernatant, Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasmaAssay typeSandwich (quantitative)Sensitivity 13.3 pg/mlRange58.8 pg/ml -2000 pg/mlRecoverySample specific recoverySample typeAverage %RangeCell culture supernatant114109%- 120%Serum113107%- 120%Cell Lysate109103%- 118%Cell culture media9391%- 95%Hep Plasma109107%- 111%EDTA Plasma113111%- 116%Cit plasma110106%- 114%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:HumanProduct overviewHuman Galectin-3 ELISA Kit (LGALS3) (ab269555) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in cell lysate, serum, cell culture media, cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-3 (LGALS3) with 13.3 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.NotesGalectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.PlatformPre-coated microplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Human Galectin-3 (LGALS3) Capture Antibody1 x 600µl10X Human Galectin-3 (LGALS3) Detector Antibody1 x 600µl10X Wash Buffer PT (ab206977)1 x 20ml5X Cell Extraction Buffer PTR (ab193970)1 x 10mlAntibody Diluent 5BI1 x 6mlHuman Galectin-3 (LGALS3) Lyophilized Recombinant Protein2 vialsPlate Seals1 unitSample Diluent NS (ab193972)1 x 100mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasNeuroscienceCell Adhesion ProteinsNuclear ProteinsCell BiologyCell CycleCell differentiationFunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.Sequence similaritiesContains 1 galectin domain.Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.Target information above from: UniProt accessionP17931The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative names35 kDa lectinCarbohydrate binding protein 35Carbohydrate-binding protein 35CBP 35CBP35Gal-3GAL3Galactose-specific lectin 3Galactoside-binding proteinGALBPGalectin 3 internal gene,includedGalectin-3Galectin3GALIGGBPIgE binding proteinIgE-binding proteinL 31L 34L-31L-34 galactoside-binding lectinL31Laminin-binding proteinLectin L-29Lectin, galactose binding, soluble 3LEG3_HUMANLGALS2LGALS3MAC 2 antigenMac-2Mac-2 antigenMAC2Macrophage galactose-specific lectinMGC105387see allDatabase linksEntrez Gene:3958 HumanOmim:153619 HumanSwissProt:P17931 HumanUnigene:531081 Human