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Abcam/Human MRP1 ELISA Kit (ABCC1) (ab272211)/1 x 96 tests/ABCC1) (ab272211
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OverviewProduct nameHuman MRP1 ELISA Kit (ABCC1)See all MRP1 kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Extract82.9%Sample typeTissue Extracts, Cell LysateAssay typeSandwich (quantitative)Sensitivity 0.151 ng/mlRange0.63 ng/ml -40 ng/mlRecoverySample specific recoverySample typeAverage %RangeTissue Extracts9594%- 96%Cell Lysate9491%- 97%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:HumanProduct overviewHumanMRP1 ELISA kit (ab272211) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement ofMRP1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. QuantitateHumanMRP1 with 151 pg/mL sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.NotesMRP1 (Multidrug Resistance-associated Protein 1), also known as ABCC1 (ATP-Binding Cassette sub-family C member 1), mediates export of organic anions and drugs from the cytoplasm. It mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. MRP1 confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.PlatformPre-coated microplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Human MRP1 (ABCC1) Capture Antibody1 x 600µl10X Human MRP1 (ABCC1) Detector Antibody1 x 600µl10X Wash Buffer PT (ab206977)1 x 20ml50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml5X Cell Extraction Buffer PTR (ab193970)1 x 10mlAntibody Diluent 4BI1 x 6mlHuman MRP1 (ABCC1) Lyophilized Recombinant Protein2 vialsPlate Seals1 unitSample Diluent NS(ab193972)1 x 12mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasSignal TransductionMetabolismPlasma MembraneChannelsSignal TransductionMetabolismDrug metabolismCancerDrug resistanceMRP-related proteinsCardiovascularAtherosclerosisHypertensionSodium/ion transportCardiovascularBloodBlood Pressure regulationCancerCancer MetabolismMetabolic signaling pathwayHormone biosynthesisMetabolismPathways and ProcessesMetabolic signaling pathwaysDrug metabolismMetabolismPathways and ProcessesEndocrine metabolismHormone biosynthesisMetabolismTypes of diseaseHeart diseaseFunctionMediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.Tissue specificityLung, testis and peripheral blood mononuclear cells.Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.Contains 2 ABC transmembrane type-1 domains.Contains 2 ABC transporter domains.Cellular localizationCell membrane.Target information above from: UniProt accessionP33527The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesABC 29ABC29ABCCABCC 1Abcc1ATP binding cassette sub family C (CFTR/MRP) member 1ATP binding cassette sub-family C member 1ATP binding cassette subfamily C member 1ATP binding cassette transporter variant ABCC1delta ex13ATP binding cassette transporter variant ABCC1delta ex13&14ATP binding cassette transporter variant ABCC1delta ex25ATP binding cassette transporter variant ABCC1delta ex25&26ATP binding cassette, sub-family C (CFTR/MRP), member 1ATP-binding cassette sub-family C member 1DKFZp686N04233DKFZp781G125GS XGSXLeukotriene C(4) transporterLTC4 transporterMRPMRP 1MRP1MRP1_HUMANMultidrug resistance associated protein 1Multidrug resistance proteinMultidrug resistance-associated protein 1Multiple drug resistance associated proteinMultiple drug resistance protein 1see allDatabase linksEntrez Gene:4363 HumanOmim:158343 HumanSwissProt:P33527 HumanUnigene:391464 Human

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