OverviewProduct nameHuman Cystatin C ELISA KitSee all Cystatin C kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%HeLa extract53.45%Inter-assaySamplenMeanSDCV%HeLa extract34.83%Sample typeCell culture supernatant, Urine, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasmaAssay typeSandwich (quantitative)Sensitivity 15 pg/mlRange39 pg/ml -2500 pg/mlRecoverySample specific recoverySample typeAverage %RangeSerum9794%- 101%Plasma9795%- 98%Cell culture media9485%- 105%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:HumanProduct overviewHuman Cystatin C ELISA kit (ab179883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in human plasma, serum urine and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Cystatin C with 15 pg/mL sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.This kit is knockout validated for specifically detectingcystatin Cin human HAP1 cells.NotesCystatin C fuctions as an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. The CST3 gene is located in the cystatin locus and comprises 3 exons (coding regions, as opposed to introns, non-coding regions within a gene), spanning 4.3 kilo-base pairs. It encodes the most abundant extracellular inhibitor of cysteine proteases. It is found in high concentrations in biological fluids and is expressed in virtually all organs of the body (CST3 is a housekeeping gene). Cystatin C has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. If kidney function and glomerular filtration rate decline, the blood levels of cystatin C rise. Serum levels of cystatin C are a more precise test of kidney function (as represented by the glomerular filtration rate, GFR) than serum creatinine levels.PlatformMicroplatePropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Human Cystatin C Capture Antibody1 x 600µl10X Human Cystatin C Detector Antibody1 x 600µl10X Wash Buffer PT (ab206977)1 x 20mlAntibody Diluent 4BI1 x 6mlHuman Cystatin C Lyophilized Recombinant Protein2 vialsPlate Seals1 unitSample Diluent NS (ab193972)1 x 50mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasCardiovascularBloodSerum ProteinsCell BiologyProteolysis / UbiquitinProtease inhibitorsOther protease inhibitorsCardiovascularAtherosclerosisThrombosisOtherKits/ Lysates/ OtherKitsELISA KitsELISA KitsCardiovascular ELISA kitsFunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.Involvement in diseaseDefects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.Sequence similaritiesBelongs to the cystatin family.Post-translationalmodificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.Cellular localizationSecreted.Target information above from: UniProt accessionP01034The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesARMD11bA218C14.4 (cystatin C)Cst3Cystatin-3Cystatin-CCYTC_HUMANGamma-traceNeuroendocrine basic polypeptidePost-gamma-globulinsee allDatabase linksEntrez Gene:1471 HumanOmim:604312 HumanSwissProt:P01034 HumanUnigene:304682 Human