OverviewProduct nameS100B ELISA KitSee all S100 beta kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Protein62.5%Sample typeTissue ExtractsAssay typeSandwich (quantitative)Sensitivity 139 pg/mlRange0.31 ng/ml -20 ng/mlRecoverySample specific recoverySample typeAverage %RangeTissue Extracts9792%- 100%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:Mouse, Rat, HumanProduct overviewS100B ELISA Kit (ab234573) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100B protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate S100B with 139 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.NotesAbcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.PlatformPre-coated microplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Wash Buffer PT (ab206977)1 x 20ml50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml5X Cell Extraction Buffer PTR (ab193970)1 x 10mlAntibody Diluent 4BI1 x 6ml10X Human S100B Capture Antibody1 x 600µl10X Human S100B Detector Antibody1 x 600µlHuman S100B Lyophilized Recombinant Protein2 vialsPlate Seals1 unitSample Diluent NS(ab193972)1 x 12mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasCell BiologyCell CycleCell differentiationEpigenetics and Nuclear SignalingCell cycleCell DifferentiationStem CellsNeural Stem CellsGlial Restricted LineageAstrocyteFunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.Tissue specificityAlthough predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.Sequence similaritiesBelongs to the S-101 family.Contains 2 EF-hand domains.Cellular localizationCytoplasm. Nucleus.Target information above from: UniProt accessionP04271The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesNEFProtein S100 BProtein S100-BS 100 calcium binding protein beta chainS 100 protein beta chainS-100 protein beta chainS-100 protein subunit betaS100S100 calcium binding protein beta (neural) S100 calcium-binding protein BS100 protein beta chainS100BS100B_HUMANS100betasee allDatabase linksEntrez Gene:6285 HumanEntrez Gene:20203 MouseEntrez Gene:25742 RatOmim:176990 HumanSwissProt:P04271 HumanSwissProt:P50114 MouseSwissProt:P04631 RatUnigene:422181 HumanUnigene:235998 MouseUnigene:8937 Ratsee all