OverviewProduct nameHuman Fibronectin ELISA KitSee all Fibronectin kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Serum35%Inter-assaySamplenMeanSDCV%Serum58.3%Sample typeCell culture supernatant, Milk, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasmaAssay typeSandwich (quantitative)Sensitivity 20.6 pg/mlRange125 pg/ml -8000 pg/mlRecoverySample specific recoverySample typeAverage %RangeCell culture supernatant10290%- 117%Milk10291%- 111%Serum9387%- 102%Cell culture extracts8980%- 105%Tissue Extracts9080%- 131%EDTA Plasma8681%- 94%Cit plasma9987%- 120%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:Human, Rhesus monkeyDoes not react with:CowProduct overviewHuman Fibronectin ELISA Kit (ab219046) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibronectin protein in cell culture supernatant, cit plasma, edta plasma, milk, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fibronectin with 20.6 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.ASSAY SPECIFICITYThis kit recognizes both native and recombinant human Fibronectin protein in serum, plasma (citrate and EDTA), milk, cell culture supernatant, and cell and tissue extract samples only.CROSS REACTIVITY Recombinant human Anastelin was prepared within the working range of the assay and assayed for cross reactivity. No cross-reactivity was observed.SPECIES REACTIVITYThis kit recognizes human Fibronectin protein.Other species reactivity was determined by measuring 1/100,000 serum (mouse, rat, cow) or plasma (Rhesus macaque) samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution.NotesFibronectin is a large glycoprotein present in the extracellular matrix and circulating plasma. Fibronectin is important in many cell adhesion and migration related processes, including wound healing, embryogenesis and nerve regeneration. Differential expression of fibronectin is seen in coronary heart disease, glomerulopathy and tumor cell metastasis. The protein contains binding sites for collagen, heparin and fibrin and is a specific ligand for several integrin adhesion receptors. Fibronectin exists as a dimer or multimer.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.PlatformPre-coated microplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Wash Buffer PT (ab206977)1 x 20ml50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml5X Cell Extraction Buffer PTR (ab193970)1 x 10mlAntibody Diluent 4BR1 x 6ml10X Human Fibronectin Capture Antibody1 x 600µl10X Human Fibronectin Detector Antibody1 x 600µlHuman Fibronectin Lyophilized Recombinant Protein2 vialsPlate Seals1 unitSample Diluent NS (ab193972)1 x 50mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasCardiovascularAngiogenesisAdhesion / ECMExtracellular MatrixSignal TransductionCytoskeleton / ECMExtracellular MatrixECM ProteinsFibronectinStem CellsLineage MarkersEctodermStem CellsNeural Stem CellsExtracellularStem CellsMesenchymal Stem CellsOsteogenesisCancerInvasion/microenvironmentECMExtracellular matrixOtherDevelopmental BiologyLineage specificationEctodermFunctionFibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.Tissue specificityPlasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.Involvement in diseaseGlomerulopathy with fibronectin deposits 2Sequence similaritiesContains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 16 fibronectin type-III domains.Developmental stageUgl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.Post-translationalmodificationsSulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylated by FAM20C in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin.Cellular localizationSecreted, extracellular space, extracellular matrix.Target information above from: UniProt accessionP02751The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesCIGCold insoluble globulin Cold-insoluble globulinDKFZp686F10164 DKFZp686H0342DKFZp686I1370DKFZp686O13149ED B Fibronectin 1FINCFINC_HUMANFNFN1FNZGFNDGFND2LETSMigration stimulating factor MSFUgl-Y3see allDatabase linksEntrez Gene:2335 HumanOmim:135600 HumanSwissProt:P02751 HumanUnigene:203717 Human