OverviewProduct nameRat PAI1 ELISA Kit (SERPINE1)See all PAI1 kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%rat serum51.6%Inter-assaySamplenMeanSDCV%rat serum33.3%Sample typeCell culture supernatant, Urine, Serum, Cit plasmaAssay typeSandwich (quantitative)Sensitivity 159 pg/mlRange298 pg/ml -5000 pg/mlRecoverySample specific recoverySample typeAverage %RangeUrine8069%- 88%Serum10094%- 105%Cell culture media9998%- 102%Cit plasma8584%- 87%serum free media9591%- 98%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:Mouse, RatProduct overviewRat PAI1 ELISA Kit (SERPINE1) (ab201283) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat PAI1 (SERPINE1) with 159 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.NotesPlasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.PlatformMicroplatePropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Rat PAI1 Capture Antibody1 x 600µl10X Rat PAI1 Detector Antibody1 x 600µl10X Wash Buffer PT (ab206977)1 x 20mlAntibody Diluent 4BI1 x 6mlPlate Seals1 unitRat PAI1 Lyophilized Recombinant Protein2 vialsSample Diluent NS (ab193972)1 x 50mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitStop Solution1 x 12mlTMBDevelopment Solution1 x 12mlResearch areasCardiovascularBloodFibrinolysis / ThrombolysisCancerInvasion/microenvironmentECMExtracellular matrixOtherCell BiologyProteolysis / UbiquitinProtease inhibitorsSerine protease inhibitorsSERPINsCell BiologyProteolysis / UbiquitinProtease inhibitorsOther protease inhibitorsCancerTumor biomarkersOtherKits/ Lysates/ OtherKitsELISA KitsELISA KitsProtease inhibitors ELISA kitsMetabolismTypes of diseaseMetabolic disordersFunctionThis inhibitor acts as "bait" for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.Sequence similaritiesBelongs to the serpin family.Post-translationalmodificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg--Met-370 bond.Cellular localizationSecreted.Target information above from: UniProt accessionP05121The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesClade EEndothelial plasminogen activator inhibitorNexin Nexin plasminogen activator inhibitor type 1PAIPAI 1PAI-1PAI1_HUMANPLANH1Plasminogen activator inhibitor 1Plasminogen activator inhibitor type 1Serine (or cysteine) proteinase inhibitor Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1Serine proteinase inhibitor clade E member 1serpinSerpin E1Serpin peptidase inhibitor clade ESerpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1Serpine 1SERPINE1see allDatabase linksEntrez Gene:18787 MouseEntrez Gene:24617 RatSwissProt:P22777 MouseSwissProt:P20961 RatUnigene:250422 MouseUnigene:29367 Rat