OverviewProduct nameHuman Hemopexin ELISA KitSee all Hemopexin kitsDetection methodColorimetricPrecisionIntra-assaySamplenMeanSDCV%Serum53.3%Inter-assaySamplenMeanSDCV%Serum34.8%Sample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay typeSandwich (quantitative)Sensitivity 370 pg/mlRange0.78 ng/ml -50 ng/mlRecoverySample specific recoverySample typeAverage %RangeCell culture supernatant107104%- 113%Serum10897%- 117%Hep Plasma10597%- 117%EDTA Plasma9485%- 103%Cit plasma9786%- 110%Assay time1h30mAssay durationOne step assaySpecies reactivityReacts with:HumanDoes not react with:CowProduct overviewHuman Hemopexin ELISA Kit (ab221838) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemopexin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemopexin with 370 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.NotesHemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer"s disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.PlatformMicroplate (12 x 8 well strips)PropertiesStorage instructionsStore at +4°C. Please refer to protocols.Components1 x 96 tests10X Human Hemopexin Capture Antibody1 x 600µl10X Human Hemopexin Detector Antibody1 x 600µlHuman Hemopexin Lyophilized Native Protein1 x 2 vialsAntibody Diluent 4BI1 x 6ml10X Wash Buffer PT (ab206977)1 x 20mlTMBDevelopment Solution1 x 12mlStop Solution1 x 12mlSample Diluent NS (ab193972)1 x 50mlSimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unitPlate Seals1 unitResearch areasSignal TransductionCytoskeleton / ECMExtracellular MatrixECM EnzymesMMPSignal TransductionMetabolismVitamins / MineralsMetabolismPathways and ProcessesCofactors, Vitamins / mineralsVitamins / mineralsFunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.Tissue specificityExpressed by the liver and secreted in plasma.Sequence similaritiesBelongs to the hemopexin family.Contains 5 hemopexin-like domains.Post-translationalmodificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.Cellular localizationSecreted.Target information above from: UniProt accessionP02790The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProtAlternative namesBeta 1B glycoprotein Beta-1B-glycoproteinFLJ56652HEMOHEMO_HUMANHemopexinHpxHXsee allDatabase linksEntrez Gene:3263 HumanOmim:142290 HumanSwissProt:P02790 HumanUnigene:426485 Human