OverviewProduct nameHuman CXCR5 knockout Raji cell lineParental Cell LineRajiOrganismHumanMutation descriptionKnockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2Passage numberKnockout validationSanger Sequencing, Western Blot (WB)Tested applicationsSuitable for:WB, Flow Cytmore detailsBiosafety level2General notesRecommended control:Human wild-type Raji cell line (ab275473). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.Cryopreservation cell medium:Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.Culture medium:RPMI+ 10% FBSInitial handling guidelines:Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80ºC. Storage at -80ºC may result in loss of viability.1. Thaw the vial in 37ºC water bath approximately 1-2 minutes.2. Transfer the cell suspension (0.8 ml) to a 15 ml/50 ml conical sterile polypropylene centrifuge tube containing 8.4 ml pre-warmedculture medium, wash vial with an additional 0.8 mlculture medium(total volume 10 ml) to collect remaining cells, and centrifuge at 201x g(rcf) for 5 minutes at room temperature. 10 ml represents minimum recommended dilution. 20 ml represents maximum recommended dilution.3. Resuspend the cell pellet in 5 ml pre-warmedculture mediumand count using a haemocytometer (Click here to view haemocytometer protocol) or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104cells/cm2. This should allow for confluency within 48 hours. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.4. Incubate the culture at 37ºC incubator with 5% CO2. Cultures should be monitored daily.Subculture guidelines:All seeding densities should be based on cell counts gained by established methods.A guide seeding density of 2x104cells/cm2is recommended for confluency (80-90% confluence) within 48 hours.A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.Cells should be passaged when they have achieved 80-90% confluence.Click here to view the Mammalian cell tissue culture protocolThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.PropertiesNumber of cells1 x 106 cells/vial, 1 mLViability~90%Adherent /SuspensionSuspensionTissueLymphaticCell typeBurkitt"s lymphomaDiseaseLymphomaGenderMaleMycoplasma freeYesStorage instructionsShipped on Dry Ice. Store in liquid nitrogen.Storage bufferConstituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl etherPurityImmunogen affinity purifiedResearch areasCardiovascularBloodOtherImmunologyAdaptive ImmunityB CellsCDImmunologyInnate ImmunityChemokinesAlpha Chemokine Rec. (CXCR)Signal TransductionSignaling PathwayG Protein SignalingGPCRCancerSignal transductionG protein signalingGPCRTargetFunctionCytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.Tissue specificityExpression in mature B-cells and Burkitt lymphoma cells.Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.Cellular localizationCell membrane.Target information above from: UniProt accessionP32302The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010Nucleic Acids Res. 38:D142-D148 (2010).Information by UniProt