Cat #: ACR-002 Alternative Name CB2, CNR2Lyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Antigen Incl. Antigen Incl.This antibody is shipped with its antigen FREE of charge!Type: PolyclonalHost: RabbitReactivity: r Immunogen - + Peptide (C)DRQVPGIARMRLDVR, corresponding to amino acid residues 228-242 of rat CB2 receptor (Accession Q9QZN9). 3rd intracellular loop.Accession (Uniprot) Number Q9QZN9Gene ID 57302Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.Homology Mouse - identical. RRID AB_2039799.Purity Affinity purified on immobilized antigen.Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.Isotype Rabbit IgG.Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.Antibody concentration after reconstitution 0.65 mg/ml.Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.Negative control antigen reconstitution 100 µl double distilled water (DDW).Negative control antigen storage after reconstitution -20°C.Preadsorption Control 1 μg peptide per 1 μg antibody.Standard quality control of each lot Western blot analysis.Applications: ih, wb Western blot - + Western blot analysis of RBL (lanes 1 and 3), C6 (lanes 2 and 4) and rat lung (lanes 5 and 6) lysates:1,2,5. Anti-Cannabinoid Receptor 2 Antibody (#ACR-002), (1:200).3,4,6. Anti-Cannabinoid Receptor 2 Antibody, preincubated with the negative control antigen. Immunohistochemistry - + Expression of CB2 receptor in rat dermisImmunohistochemical staining of paraffin embedded section of rat dermis using Anti-Cannabinoid Receptor 2 Antibody (#ACR-002), (1:100). CB2 is expressed in sebaceous glands and ducts of sweat glands in the reticular dermis. Hematoxilin is used as the counterstain. References1. Brooks, J.W. and Fraquhar-Smith, M.A. (2003) Br. J. Anaesth. 3, 175.2. Howlett, A.C. (2002) Prostaglandins Other Lipid Mediat. 68, 619.3. Guo, J. and Ikeda, S.R. (2004) Mol. Pharmacol. 65, 665.4. Wotherspoon, G. et al. (2005) Neuroscience 135, 235.5. Fernandez-Ruiz, J. et al. (2007) Trends Pharmacol. Sci. 28, 39.6. Sarfaraz, S. et al. (2005) Cancer Res. 65, 1635.7. Sánchez, C. et al. (2001) Cancer Res. 61, 5784. Scientific background - + Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 receptor is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in the brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relationship between CB2 expression and tumor malignancy was demonstrated.8 Activation of CB2 in vivo by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in the internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.Application key:CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry,IE- Indirect ELISA, IFC- Indirect flow cytometry,IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation,LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key: H- Human, M- Mouse, R- RatLyophilized PowderNegative Control Antigen IncludedCertificate of Analysis MSDSLast update: 09/09/2019Alomone is pleased to offer a new antibody directed against an intracellular epitope of the rat CB2 receptor. Anti-Cannabinoid Receptor 2 Antibody (#ACR-002) can be used in western blot and immunohistochemistry applications, and recognizes CB2 in rat samples.For research purposes only, not for human use