Cat #: ACL-021 Alternative Name Cl- intracellular channel 1, Nuclear chloride ion channel 27, NCC27, Chloride channel ABPLyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Antigen Incl. Antigen Incl.This antibody is shipped with its antigen FREE of charge!Type: PolyclonalHost: RabbitReactivity: h, m, r Immunogen - + Peptide (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299). Intracellular, C-terminus.Accession (Uniprot) Number O00299Gene ID 1192Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.Homology Rat, mouse - identical. RRID AB_11122892.Purity Affinity purified on immobilized antigen.Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.Isotype Rabbit IgG.Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.Antibody concentration after reconstitution 0.8 mg/ml.Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.Negative control antigen reconstitution 100 µl double distilled water (DDW).Negative control antigen storage after reconstitution -20°C.Preadsorption Control 1 µg peptide per 1 µg antibody.Standard quality control of each lot Western blot analysis.Applications: ic, if, wbMay also work in: ifc*, ih*, ip* Western blot - + Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:1-4. Anti-CLIC1 Antibody (#ACL-021), (1:200).5-8. Anti-CLIC1 Antibody, preincubated with the negative control antigen. Immunocytochemistry - + Expression of CLIC1 in human colorectal adenocarcinoma HT-29 cellsImmunocytochemical staining of fixed and permeabilized human colorectal adenocarcinoma HT-29 cells. A. Cells were stained with Anti-CLIC1 Antibody (#ACL-021), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images. References1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102.2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1.3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356.4. Ulmasov, B. et al. (2007) BMC Cell. Biol. 8, 8.5. Jung, J.J. and Kitajewski, J. (2010) J. Angiogenes. Res. 2, 23. Scientific background - + Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1.CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5.Application key:CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry,IE- Indirect ELISA, IFC- Indirect flow cytometry,IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation,LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key: H- Human, M- Mouse, R- RatLyophilized PowderNegative Control Antigen IncludedClick here to receivea 50 µl free trial sample!Certificate of Analysis MSDSLast update: 10/11/2019Anti-CLIC1 Antibody (#ACL-021) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC1 from mouse, rat, and human samples.For research purposes only, not for human use