首页 > Discovery > Discovery Antibodies/Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody/0.2 ml/AGP-009-0.2 ml

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Discovery Antibodies/Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody/0.2 ml/AGP-009-0.2 ml

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AGP-009-0.2ml

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Cat#:AGP-009AlternativeNameAMPAReceptor1,Glutamatereceptor1,Ionotropicglutamatereceptor1,AMPA-selectiveglutamatereceptor1,GRIA1, GluR-A, GluR-K1LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!Type:PolyclonalHost:GuineapigReactivity:h,m,rImmunogen-+PeptideRTSDSRDHTRVDWKR(C),correspondingtoaminoacidresidues271-285ofratGluR1(Accession P19490).Extracellular,N-terminus.Accession(Uniprot)NumberP19490GeneID50592PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyMouse-identical;human,pig,dog-13/14aminoacidresiduesidentical.RRIDAB_2340961.PurityAffinitypurifiedonimmobilizedantigen.FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.IsotypeGuineapigtotalIgG.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.8mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).Negativecontrolantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ic,if,ih,lci,wbMayalsoworkin:ifc*,ip*Westernblot-+Westernblotanalysis ofrat(lanes1and3)andmouse(lanes2and4)brainlysates:1,2.Guineapig Anti-GluR1(GluA1)(extracellular) Antibody(#AGP-009), (1:200).3,4.GuineapigAnti-GluR1(GluA1)(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.Immunohistochemistry-+ExpressionofGluR1inrathippocampusImmunohistochemicalstainingofperfusion-fixedfrozenratbrainsections usingGuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009),(1:400),followedbyanti-rabbit-Cy2antibody(green).GluR1stainingappearsinneuronaloutlines(horizontalarrows)andintheinnermolecularlayerofthedentategyrus(verticalarrow).NucleiarestainedwithDAPI(blue).Livecellimaging/Immunocytochemistry-+Immuno-colocalizationofGluR1andVesicularGABATransporterinhumanU-87MGcellsCellsurfacedetectionof GluR1andVesicularGABATransporterinhumanglioblastomaU-87MG.ExtracellularstainingofliveintactcellswithGuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009),(1:25),followedbygoatanti-guineapig-AlexaFluor-488secondaryantibody(green).Cellsweresubsequentlyfixed,permeABIlizedandlabeledwith Anti-VesicularGABATransporter(VGAT)Antibody(#AGT-005),(1:200),followedbygoatanti-rabbit-AlexaFluor-594secondaryantibody(red).RepresentativemergedimagesofthedoublelabeledcellsareshowninAandB.References1.Dingledine,R.etal.(1999)Pharmacol.Rev.51,7.2.Sheng,M.etal.(2001)Cell.105,825.3.Song,I.etal.(2002)Trends.Neurosci.25,578.Scientificbackground-+AMPAreceptors aremembersofthe glutamatereceptorfamily ofionchannelsthatalsoincludetheNMDAand Kainate receptors.ThethreesubfamiliesarenamedaftertheoriginalsyntheticagoNISTsthatwereidentifiedasselectiveligandsofeachfamily.Theα-amino-3-hydroxy-5-methyl-4-isoazolepropionicacid(AMPA)receptorsubfamilyincludesfourmembersAMPA1-AMPA4thatarealsoknownasGluR1-GluR4respectively.ThefunctionalAMPAchannelisbelievedtobeatetramer,withmostneuronalAMPAreceptorsbeingactuallyheterotetramerscomposedofAMPA1plus AMPA2 orAMPA2plus AMPA3,althoughhomotetramerscanalsobefound.AMPAreceptorsarepermeabletocationsNa+,K+ andCa2+.TheCa2+ permeabilityisdependentonthepresenceofAMPA2:wheneverthissubunitispresent,thechannelwillbeimpermeabletoCa2+.TheCa2+ permeabilityoftheAMPA2subunitisdeterminedbythepresenceofanarginine(R)atacriticalsiteintheporeloopinsteadofaglutamine(Q)presentinthesamesiteintheotherAMPAsubunits.Apost-transcriptionalprocessknownasRNAeditingdeterminesthepresenceofthisR.SincemostAMPA2subunitsintheadultbrainhaveundergoneRNAeditingandmostAMPAreceptorscontaintheAMPA2subunit, mostnativeAMPAreceptorswillbeimpermeabletoCa2+.GatingofAMPAreceptorsbyglutamateisextremelyfastandthereforetheAMPAreceptorsmediatemostexcitatory(depolarizing)currentsinthebrainduringbasalneuronalactivity.Thedepolarizationcausedbytheactivationofpost-synapticAMPAreceptorsisnecessaryfortheactivationofNMDAreceptorsthatwillopenonlyinthepresenceofbothglutamateandadepolarizedmembrane.Synapticstrength,definedasthelevelofpost-synapticdepolarization,canbelongterm(hencethetermlongtermpotentiation,LTP)andthereforeinducechangesinsignalingandproteinsynthesisintheactivatedneuron.Thesechangesareassociatedwithmemoryformationandlearning.ChangesinsynapticstrengtharethoughttoinvolverapidmovementoftheAMPAreceptorsinandoutofthesynapsesandagreatdealofefforthasfocusedinunderstandingthemechanismsthatgovernAMPAreceptortrafficking.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IF-Immunofluorescence,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderNegativeControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSImage&Title:ExpressionofGluR1inmouseolfactorybulb.ImmunohistochemicalstainingofmouseolfactorybulbsectionsusingGuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009).GluR1staining(green)isdetectedintheglomerularandexternalplexiformlayers(EPL),(rightpanel).GluR1co-localizeswithGFAPinperiglomerularastrocytesandtheirprocessesintheneuropil(mergedpanel).Adaptedfrom Droste,D. etal. (2017) Sci.Rep. 7, 44817.withpermissionofNaturePublishingGroup.Lastupdate:11/11/2019AlomoneLabsispleasedtoofferanantibodyagainstanextracellularepitopetherationotropicglutamatereceptor1.GuineapigAnti-GluR1(GluA1)(extracellular)Antibody (#AGP-009)raisedinguineapigcanbeusedinwesternblot,immunohistochemistryandimmunocytochemistryapplications.IthasbeendesignedtorecognizeGluR1fromhuman,mouseandratsamples.TheantigenusedtoimmunizeguineapigsisthesameasAnti-GluR1(GluA1)(extracellular)Antibody(#AGC-004)raisedinrabbit.Ourlineofguineapigantibodiesenablesmoreflexibilitywithourproductssuchasimmuno-colocalizationstudies,immunoprecipitation,etc.Forresearchpurposesonly,notforhumanuse

Discovery®抗体是剑桥生物化学研究所（CRB）的新目录抗体业务。

CRB是世界上第二大多肽公司，也是抗体研究市场的领跑者，从目录业务到现在已有22年的休息期（1994-2016年），现在是第37年（2017年），CRB推出了一个全新的现成抗体业务，名为Discovery®抗体。

Discovery®抗体将专注于研究抗体（早期药物“Discovery”），并将补充我们非常成功和长期的定制抗体业务(www.crbdiscovery.com网站). 我们以新颖、复杂、稀有和翻译后修饰的特异性抗体而闻名，这些备受追捧的目标将出现在我们的发现®抗体目录范围内。

我们于2017年2月推出了我们的发现®抗体，一年后发现®肽，大约240年后，詹姆斯·库克船长（他那个时代最伟大的探险家）于1776年从英国乘“发现”号船出发，包租全球的新水域和“发现”新大陆。因此，“发现”这个名字似乎适合东北部的一家制造商——詹姆斯·库克的家乡。

在17世纪末对世界各地新发现的土地的探索和2000年医学研究的新“发现”以及人类基因组的绘制之间产生了进一步的相似之处。

有关更多详情，请参阅我们发布的Discovery®抗体的新闻稿

剑桥研究生物化学(www.crbdiscovery.com网站)是一家领先的定制肽和抗体工具的独立生产商，为全球制药、生命科学和学术领域的研究人员提供服务。其产品的主要用途是作为定制的实验室级试剂，用于证明早期药物发现中的主要研究，并支持临床研究中的生物标记物方案。该公司生产各种口味的肽，从简单到改性，稳定，从毫克到克标到最高纯度的染料标签。

Discovery®抗体是剑桥生物化学研究所（CRB）的新抗体目录业务