Cat #: ACC-116 Alternative Name Inositol 1,4,5-trisphosphate receptor type 2, IP3R2, InsP3R2, IP3 receptor isoform 2Lyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Antigen Incl. Antigen Incl.This antibody is shipped with its antigen FREE of charge!Type: PolyclonalHost: RabbitReactivity: rMay also work in: h*, m* Immunogen - + Peptide (C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2 (Accession P29995). Intracellular, C-terminus.Accession (Uniprot) Number P29995Gene ID 81678Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.Homology Mouse, human - 13/14 amino acid residues identical. RRID AB_2340910.Purity Affinity purified on immobilized antigen.Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.Isotype Rabbit IgG.Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.Antibody concentration after reconstitution 0.8 mg/ml.Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.Negative control antigen reconstitution 100 µl double distilled water (DDW).Negative control antigen storage after reconstitution -20°C.Preadsorption Control 1 µg peptide per 1 µg antibody.Standard quality control of each lot Western blot analysis.Applications: if, ih, wbMay also work in: ic*, ifc*, ip* Western blot - + Western blot analysis of rat brain membrane (lanes 1 and 3) and rat basophilic leukemia (RBL) cell line lysate (lanes 2 and 4):1,2. Anti-IP3 Receptor-2 (ITPR2) Antibody (#ACC-116), (1:200).3,4. Anti-IP3 Receptor-2 (ITPR2) Antibody, preincubated with the negative control antigen. Immunohistochemistry - + Expression of IP3R2 in rat spinal cordImmunohistochemical staining of IP3R2 in rat spinal cord sections using Anti-IP3 Receptor-2 (ITPR2) Antibody (#ACC-116). A. IP3R2 immunoreactivity (red) appears in neuronal soma (horizontal arrows) and processes (vertical arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B. References1. Furuichi, T. et al. (1989) Nature 342, 32.2. Berridge, M.J. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 517.3. Furuichi, T. et al. (1994) Curr. Opin. Neurobiol. 4, 294.4. Varnai, P. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 7859.5. Fujino, I. et al. (1995) Cell Tissue Res. 280, 201.6. Yaroslavskiy, B.B. et al. (2007) J. Cell Sci. 120, 2884. Scientific background - + Inositol 1,4,5-trisphosphate receptors (IP3R) are intracellular Ca2+ release channels located on the endoplasmic reticulum (ER) and mediate Ca2+ mobilization from the ER to the cytoplasm in response to the binding of the second messenger, inositol 1,4,5-trisphosphate (IP3)1. IP3-induced Ca2+ release is triggered by various external stimuli, and most non-excitable cells use this mechanism as the primary Ca2+ signaling pathway. IP3Rs are therefore thought to have important physiological roles in various cell types and tissues2.Three subtypes of IP3Rs, derived from three distinct genes, have been identified in mammals3. All three receptors have six transmembrane domains and a pore region between TM5 and TM6. The N-terminus as well as the C-terminus are cytoplasmic. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required to maintain the suppression of channel activity4. Type 2 IP3R (IP3R2) is expressed in various tissues and cell lines. IP3R2 mRNA is localized in the intralobular duct cells of the submandibular gland, the urinary tubule cells of the kidney, the epithelial cells of epididymal ducts and the follicular granulosa cells of the ovary5. IP3R2 is active during osteoclast differentiation, and its absence causes a partial defect in osteoclastic differentiation.Application key: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key: H- Human, M- Mouse, R- RatLyophilized PowderNegative Control Antigen IncludedClick here to receive a 25 µl free trial sample! Certificate of Analysis MSDSLast update: 10/11/2019Anti-IP3 Receptor-2 (ITPR2) Antibody (#ACC-116) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IP3R2 from rat, mouse and human samples.For research purposes only, not for human use