首页 > Discovery > Discovery Antibodies/A23187/10 mg/A-600-10 mg

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Discovery Antibodies/A23187/10 mg/A-600-10 mg

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A-600-10mg

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Cat #: A-600 Alternative Name Calcimycin, Calcium ionophore A23187Lyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Bioassay Tested Bioassay TestedEvery lot is tried & tested in a relevant biological assay. Our BioassayOrigin Streptomyces chartreusis.Source NaturalMW: 523.6Purity: >98%Effective concentration 1-10 µM. Structure - + Chemical name 5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11- trimethy l-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7- dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid.Molecular formula C29H37N3O6.CAS No.: 52665-69-7.Activity A23187 is a mobile-carrier Ca2+ ionophore and can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors1,2. Furthermore, A23187 induces, in a concentration- and time-dependent manner, Ca2+ triggered cell apoptosis3,4. References-Activity - + Berridge, M.J. (1993) Nature 361, 315.Scharff, O. et al. (1993) Physiol. Rev. 73, 547.Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.Hajnoczky, G. et al. (2003) Biochem. Biophys. Res. Commun. 304, 445.Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.Solubility DMSO, ethanol or methanol. Centrifuge all product preparations before use (10000 x g 5 min).Storage of solutions Up to one week at 4°C or three months at -20°C.Protect from light. Our bioassay - + Alomone Labs A23187 induces apoptosis in Jurkat cells.2 µM A23187 (#A-600) or vehicle was added to the confluent cell culture for 6 hours. Apoptosis was probed by the detection of cleaved Caspase 3 using Caspase 3 specific antibodies in western blot analysis of cell lysates. References - Scientific background1. Berridge, M.J. (1993) Nature 361, 315.2. Scharff, O. et al. (1993) Physiol. Rev. 73, 547.3. Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552.4. Hajnoczky, G. et al. (2003) Biochem. Biophys. Res. Commun. 304, 445.5. Caron-Leslie, L.A. et al. (1991) Mol. Endocrinol. 5, 1169.6. Caron-Leslie, L.A. et al. (1994) FASEB J. 8, 639.7. Rzigalinski, B.A. et al. (1994) Biochim. Biophys. Acta. 1223, 219.8. Vrana, J.A. et al. (1998) Int. J. Oncol. 12, 927.9. Petersen, A. et al. (2000) Brain Res. 857, 20.10. Cheng, C.H. et al. (2002) FEBS Lett. 516, 191.11. Jansen, S. et al. (2003) Biol. Trace Elem. Res. 96, 133. Scientific background - + A23187 is a mobile-carrier Ca2+ ionophore (allows Ca2+ ions to cross cell membranes) originally isolated as an antibiotic from Streptomyces chartreusensis. It is used to increase intracellular Ca2+ levels and for in vitro studies related to intracellular Ca2+-mediated events. A23187 can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors.1,2Furthermore, A23187 induces, in a concentration- and time-dependent manner, Ca2+-triggered cell apoptosis.3,4 This property was exploited to clarify apoptotic events in different cell lines such as mouse lymphoma cell line (S49),5,6 histiocytic lymphoma U937,7 human myeloid leukemia cells (HL-60),8 cultured striatal neurons,9 renal tubular cells,10 and C6 glioma cells.11Lyophilized PowderClick here to receive a 1 mg free trial sample! Certificate of AnalysisLast update: 25/09/2019A23187 (#A-600) is a highly pure, natural, and biologically active compound.For research purposes only, not for human use

Discovery®抗体是剑桥生物化学研究所（CRB）的新目录抗体业务。

CRB是世界上第二大多肽公司，也是抗体研究市场的领跑者，从目录业务到现在已有22年的休息期（1994-2016年），现在是第37年（2017年），CRB推出了一个全新的现成抗体业务，名为Discovery®抗体。

Discovery®抗体将专注于研究抗体（早期药物“Discovery”），并将补充我们非常成功和长期的定制抗体业务(www.crbdiscovery.com网站). 我们以新颖、复杂、稀有和翻译后修饰的特异性抗体而闻名，这些备受追捧的目标将出现在我们的发现®抗体目录范围内。

我们于2017年2月推出了我们的发现®抗体，一年后发现®肽，大约240年后，詹姆斯·库克船长（他那个时代最伟大的探险家）于1776年从英国乘“发现”号船出发，包租全球的新水域和“发现”新大陆。因此，“发现”这个名字似乎适合东北部的一家制造商——詹姆斯·库克的家乡。

在17世纪末对世界各地新发现的土地的探索和2000年医学研究的新“发现”以及人类基因组的绘制之间产生了进一步的相似之处。

有关更多详情，请参阅我们发布的Discovery®抗体的新闻稿

剑桥研究生物化学(www.crbdiscovery.com网站)是一家领先的定制肽和抗体工具的独立生产商，为全球制药、生命科学和学术领域的研究人员提供服务。其产品的主要用途是作为定制的实验室级试剂，用于证明早期药物发现中的主要研究，并支持临床研究中的生物标记物方案。该公司生产各种口味的肽，从简单到改性，稳定，从毫克到克标到最高纯度的染料标签。

Discovery®抗体是剑桥生物化学研究所（CRB）的新抗体目录业务