Cat #: ACC-021-AR Alternative Name Voltage-dependent T-type calcium channel subunit α1GKO Validated KO Validated The specificity of this antibody has been validated in a knockout (KO) or knockdown (KD) system.Lyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Antigen Incl. Antigen Incl.This antibody is shipped with its antigen FREE of charge!Type: PolyclonalHost: RabbitReactivity: h, m, r Immunogen - + Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G (Accession O54898). Intracellular, N-terminus.Accession (Uniprot) Number O54898Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.Homology Mouse - identical; human - 20/22 amino acid residues identical. RRID AB_2340896.Purity Affinity purified on immobilized antigen.Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.Isotype Rabbit IgG.Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.Reconstitution 50 µl double distilled water (DDW).Antibody concentration after reconstitution 1 mg/ml.Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.Negative control antigen reconstitution 100 µl double distilled water (DDW).Negative control antigen storage after reconstitution -20°C.Preadsorption Control 1 µg peptide per 1 µg antibody.Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-021), and immunohistochemistry (labeled antibody).Applications: if, ihMay also work in: ic* Immunohistochemistry - + Expression of CACNA1G in rat and mouse cerebellumImmunohistochemical staining of mouse (A) and rat (B) cerebellum using Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody (#ACC-021-AR). A, B. CACNA1G staining (red) appears in Purkinje neurons (arrow) and in the molecular layer (Mol). Nuclei were stained using DAPI as the counterstain (Blue). References1. Serrano, J.R. et al. (1999) J. Gen. Physiol. 114, 185.2. Perez-Reyes, E. (2003) Physiol. Rev. 83, 117.3. Wain, H.M. et al. (2002) Nucleic Acids Res. 30, 169.4. Andreasen, D. et al. (2000) Am. J. Physiol. 279, F997. Scientific background - + Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1 T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the CaV channel family.2-3 The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms.  CACNA1G (CaV3.1) and CACNA1H (CaV3.2) are widely distributed whereas the expression of CACNA1I (CaV3.3) is restricted to the central nervous system.2CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.Application key: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key: H- Human, M- Mouse, R- RatLyophilized PowderNegative Control Antigen Included Certificate of Analysis MSDSLast update: 10/11/2019Anti-CACNA1G (CaV3.1) Antibody (#ACC-021) is a highly specific antibody directed against an epitope of the rat CaV3.1 channel. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CACNA1G from human, rat, and mouse samples.Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody (#ACC-021-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CACNA1G (CaV3.1)-ATTO-594 Antibody is ideal for immuno-colocalization studies.For research purposes only, not for human use