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Biosensis/Rabbit polyclonal antibody to DJ-1/PARK7: IgG/R-1682-500/500 ug
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产品说明
Description
Autosomal recessive mutations in DJ-1 cause early-onset familial Parkinson's disease. DJ-1 is considered a redox-sensitive cytoplasmic protein found in brain as well as other cell types.
Batch No.
See product label
Unit size
500 ug
Antigen
A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1 [2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Antibody Type
Polyclonal
Other Names
PARK7
Produced in
Rabbit
Applications
WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
Specificity
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption.
Species Against
Human and mouse DJ-1 are highly conserved, so cross-reactivity with other species is expected.
Form
Lyophilized from PBS, pH 7.4. Contains no preservative.
Reconstitution
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Storage
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Expiry Date
12 months after purchase
General References
Sonia George, Su San Mok, Milawaty Nurjono, Scott Ayton, David I. Finkelstein, Colin L. Masters,Qiao-Xin Li & Janetta G. Culvenor (2010) alpha-Synuclein Transgenic Mice Reveal Compensatory Increases in Parkinson's Disease-Associated Proteins DJ-1and Parkin and Have Enhanced alpha-Synuclein and PINK1 Levels After Rotenone Treatment J Mol Neurosci 42:243_254
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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