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产品说明
Description
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Batch No.
See product label
Unit size
50 µl
Antigen
Purified bovine NF-H. Bovine intermediate filaments were prepared from spinal cords and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis.
Antibody Type
Antiserum
Other Names
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession
P12036 NFH_HUMAN;
Produced in
Rabbit
Applications
Western Blotting (WB), ELISA and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity
The specificity of this antibody has been confirmed by WB. This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa.
Antibody Against
Neurofilament Heavy, phosphorylated
Cross-reactivity
Rat. Predicted to react with other mammals due to sequence homology.
Blast URL
Click here
Form
Lyophilised
Appearance
White powder
Reconstitution
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage
After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date
12 months after purchase
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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