首页 > Biosensis > Biosensis/Rabbit polyclonal antibody to human Growth-associated protein 43 (216-238): Affinity purified/R-1055-100/100 µg

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Biosensis/Rabbit polyclonal antibody to human Growth-associated protein 43 (216-238): Affinity purified/R-1055-100/100 µg

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R-1055-100

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DescriptionGrowth-associatedprotein43(GAP-43)isexpressedbydevelopingandregeneratingneurons,andtoalesserextent,reactiveglialcells. Itisalsoamajorcomponentofneuronalgrowthcones.GAP-43bindscalmodulinwithagreateraffinityintheabsenceofCa(2+)thaninitspresence.PhosphorylationofGAP-43byaproteinKinaseCiscorrelatedwithcertainformsofsynapticplasticity.GAP-43isusedtospecificallylabelinjuredneuronsandtoscoreneuronalregeneration.BatchNo.SeeproductlabelUnitsize100µgAntigenAsyntheticpeptidecorrespondingtoaregion(216-238)fromhumanGrowth-associatedprotein43(GAP-43).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA.OtherNamesNeuromodulin;GAP43;PP46;p57;B-50;F1;AxonalmembraneproteinGAP-43;Growth-associatedprotein43;NeuralphosphoproteinB-50;AccessionP17677NEUM_HUMANProducedinRabbitPurityAffinitypurifiedonantigencolumnApplicationsImmunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanGAP-43hasapredictedlengthof238residuesandMWof25kDa.Aconcentrationof0.5-1µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrecommended.SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen.Cross-reactivityHuman;rat;predictedtoreactwithmouse;BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.FormLyophilizedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3addedaspreservativeReconstitutionReconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcyclesExpiryDate12monthsafterpurchase

Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。