首页 > Biosensis > Biosensis/Rabbit polyclonal antibody to Nicastrin, central region: IgG/R-1685-500/500 ug

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

Biosensis/Rabbit polyclonal antibody to Nicastrin, central region: IgG/R-1685-500/500 ug

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

R-1685-500

品牌：

Biosensis

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

DescriptionNicastrin,atype1membraneglycoprotein,isanessentialcomponentofthegammasecretasecomplexwhichiscriticalforthecleavageoftheamyloidprecursorproteinandothermembraneproteins.Nicastriniswidelyexpressedindifferenttissuetypes.ThisantibodyreactswithimmatureformsofNicastrin.Detectionofhighermol.wt.matureformsislikelytobeblockedbyglycosylationinthisregionoftheprotein.BatchNo.SeeviallabelUnitsize500ugAntigenAsyntheticpeptide(C-QGETFDYIGSSRMVYD)correspondingtohumanNicastrin[331-346]inthecentralregionconjugatedviaadditionalN-terminalCystoDiphtheriatoxoid.AntibodyTypePolyclonalProducedinRabbitApplicationsWBandIP.Suggestedconcentrationof3-10ug/mLisrecommendedforWB.Humanormousebrainsamplescommonlypreparedwithreducing(50mMDTT),urea(2.3M),SDS(1.5%)in62.5mMTris-HCLpH6.8samplebuffer(withoutboiling)heatingto50Cfor15min.UnprocessedfulllengthhumanNicastrinis709aminoacids,howeverthisproteincontainsanN-terminalsignalpeptidewhichisconsideredtoundergoremoval/cleavageduringprocessingandtransittothecellplasmamembrane,inadditiontheproteinundergoesglycosylationtoproduceaglycoproteinofabout145kDaapparentMWbySDSPAGE.Biosensisrecommendsthattheoptimalworkingdilutionshouldbedeterminedbytheenduser.SpecificityConfirmedbyWBusingpeptideabsorption.SpeciesAgainstHuman,mouse,ratandguineapig.Nicastrinishighlyconserved,socross-reactivitywithotherspeciesisexpected.FormLyophilizedfromPBS,pH7.4.Containsnopreservative.ReconstitutionReconstitutein500µLofsterilewater.Centrifugetoremoveanyinsolublematerial.StorageShorttermstorageat2-8°Cforoneweek.At-20°Casanundilutedliquidforupto12months.ExpiryDate12monthsafterpurchaseReferences1.Culvenor,J.G.,Ilaya,N.T.,Ryan,M.T.,Canterford,L.,Hoke,D.,Williamson,N.A.,McLean,C.A.,Masters,C.L.,and1.Evin,G.(2004)CharacterizationofPresenilincomplexfrommouseandhumanbrainusingBlueNativegelelectrophoresisrevealshighexpressioninembryonicbrainandminimalchangeincomplexmobilitywithPresenilinmutations.Eur.J.Biochem.271,375-385.2.Ilaya,N.T.,Evin,G.,Masters,C.L.,andCulvenor,J.G.(2004)Nicastrinexpressioninmouseperipheraltissuesisnotco-ordinatedwithPresenilinandishighinmuscle.J.NeuRochem.91,230-237.

Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。