首页 > Biosensis > Biosensis/Neurotrophin Antibody Kit (Trial Size)/KIT-NT-100/5 vials, each containing 100 μg sheep IgG

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Biosensis/Neurotrophin Antibody Kit (Trial Size)/KIT-NT-100/5 vials, each containing 100 μg sheep IgG

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KIT-NT-100

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Biosensis

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DescriptionNeurotrophinsaregrowthfactorsthatregulateneuronaldevelopment,maintenanceandplasticity.Thesehomo-dimericproteinsactivateamultitudeofintracellularsignallingcascadesthroughtheirinteractionwithTrkreceptorsandp75NTR.TheNeurotrophinAntibodyKit(TrialSize)containsacollectionofBiosensispopularsheepantibodiesraisedagainstBDNF(S-015-500),NGF(S-052-500),NT3(S-057-500)andNT4/5(S-059-500).ThekitiscompletedwithonevialofnormalsheepIgG(S-1754-100)foruseasnegativecontrol.ThistrialsizeofBiosensisNeurotrophinAntibodyKitcontains100μgsheepIgGofeachantibody.Thiskitpresentsacost-effectivewaytoinvestigateneurotrophinproteinexpressionandfunctionbyimmunologicaltechniquessuchasimmunohistochemistry,ELISA,andinhibitionofBIOLOGicalactivity.RelatedproductsS-015-500,SheeppolyclonalantibodytorhBDNFS-052-500,SheeppolyclonalantibodytobetaNGFS-057-500,SheeppolyclonalantibodytorhNT3S-059-500,SheeppolyclonalantibodytorhNT4/5S-1754-500,NormalsheepIgGfromnon-immunizedanimalBatchNo.Seeproductlabel.Unitsize5vials,eachcontaining100μgsheepIgGAntigenBDNF:recombinanthumanBDNFNGF:nativemousebetaNGFpurifiedfromsuBMAxillarysalivarygland(95%puritybyPAGE)NT3:recombinanthumanNT3NT4/5:recombinanthumanNT4AntibodyTypePolyclonal.IsotypeIgGAccessionP23560BDNF_HUMAN;P01139NGF_MOUSE;P20783NTF3_HUMAN;P34130NTF4_HUMAN;ProducedinSheep.PurityProteinGpurifiedIgG.ApplicationsImmunohistochemistry:1-10µg/mL.BDNFantibodyworksbestonZamboni"sfixed,frozentissue,andisnotrecommendedforparaffin-embeddedtissue.ELISA:1-10µg/mL.WesternBlotting:1-10µg/mL.WesternblottingisnotrecommendedfortheBDNFantibody.Inhibitionofbiologicalactivityinvitro/invivo:ExpectedED50(invivo)valuesareintherangeof2-10µg/mL.TheNGFantibodycompletelyinhibitsneuronalsurvivalandtheoutgrowthactionsofmurineNGFinchickenDRGinvitro.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.Thenegativecontrol(normalsheepIgG)shouldbeusedatthesameconcentrationastheneurotrophinantibodies.SpecificityBDNFAntibody:Across-reactivityoflessthan1%againstmouseNGF,recombinanthumanNT3orNT4/5hasbeenshownbyELISA.NGFAntibody:Across-reactivityoflessthan1%againstrecombinanthumanBDNF,NT3andNT4/5byELISAhasbeenshown.NT3Antibody:Across-reactivityoflessthan1%tomouseNGF,recombinanthumanBDNFand5%toNT4/5hasbeenshownbyELISA.NT4/5Antibody:Lessthan1%cross-reactivityagainstNGF,recombinanthumanBDNFand5%toNT3hasbeenshownbydotblot.SpeciesAgainstHuman(BDNF,NT3andNT4/5).Mouse(NGF).Cross-reactivityNeurotrophinssharealargedegreeofhomologyamonghuman,rodentandothermammalianspecies,thustheseneurotrophinantibodiesareusefulforanumberofspecies,includinghuman,mouseandrat.FormLyophilizedfromPBS,pH7.4,withoutpreservatives.AppearanceWhitepowder.ReconstitutionReconstituteeachvialwith100µLsterile-filtered,ultrapurewater,toachieveanantibodyconcentrationof1mg/mL.Centrifugebrieflytoremoveanyinsolublematerial.StorageStorelyophilised,unopenedvialsat2-8°Corlower.Afterreconstitution,preparealiquotsandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.ExpiryDate12monthsafterpurchase(unopenedvials).

Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。