首页 > Biosensis > Biosensis/Rabbit polyclonal antibody to glutathione peroxidase 1 (GPx-1) [175-194]: whole serum/R-1588-100/100 µl

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Biosensis/Rabbit polyclonal antibody to glutathione peroxidase 1 (GPx-1) [175-194]: whole serum/R-1588-100/100 µl

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R-1588-100

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DescriptionGlutathioneperoxidase1hasaroleindetoxificationofhydrogenperoxideandisoneofthemostimportantantioxidantenzymesinhumans.Itexistsasahomotetramerwhichlocalisestothecytoplasm.Itbelongstotheglutathioneperoxidasefamily.Glutathioneperoxidase1isoneoffewproteinsinhighervertebratestocontainselenocysteine,whichoccursattheactivesiteofglutathioneperoxidaseandiscodedbyUGA,thatnormallyfunctionsasatranslationterminationcodon.ThisproteinhasapolyalaninesequencepolymorphismintheN-terminalregion,whichincludesthreealleleswithfive,sixorsevenalaninerepeats.Theallelewithfivealaninerepeatsissignificantlyassociatedwithbreastcancerrisk.Atleasttwoalternativelysplicedisoformshavebeenidentified.BatchNo.SeeproductlabelUnitsize100µlAntigenAsyntheticpeptide(RRYSRRFQTIDIEPDIEALL)correspondingtotheaminoacids175-194ofhumanglutathioneperoxidase1(GPx-1)conjugatedtodiphtheriatoxinhasbeenusedastheimmunogen.AntibodyTypePolyclonalOtherNamesGlutathioneperoxidase1;GPx-1;GSHPx-1;Cellularglutathioneperoxidase;GPX1;AccessionP07203GPX1_HUMAN;ProducedinRabbitApplicationsIHC,WBandELISA.ThisantibodyworkssuperblyinIHConfrozenorwaxembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.TypicalworkingdilutionsforroutineIHCare1:200to1:1,000dependingontissueanddetectionmethod.ForWBadilutionrangeof1:1,000to1:4,000isrecommended.ForELISA,adilutionof1:1,000to1:4,000isrecommended.Thisantiserumhasextremelyhightitre,itstainshumanandratglialcellsintenselyandtosomeextentalsostainsneurons.Othertissueshavenotyetbeentested.OnWBunderreducingconditionsrecognisesa22kDproteininhumanbraintissueandSigmaglutathioneperoxidase(G-4013)isolatedfromhumanerythrocytes.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.SpecificityHumanandratcellularseleniumdependentglutathioneperoxidase.Cross-reactivityHuman;rat;BlastURLClickhereFormLyophilizedserumwith0.02%thimerosalReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.

Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。