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Biosensis/Rabbit polyclonal antibody to human glutathione S-transferase pi (197-210): Affinity purified/R-1060-100/100 µg
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DescriptionGlutathioneStransferases(GSTs)arealargefamilyofenzymeswhichcatalysetheconjugationofreducedglutathionetoelectrophilicsubstrates.GlutathioneS-transferasepiisoneoffourmajortypesofmammalianGSTs.BatchNo.SeeproductlabelUnitsize100µgAntigenAsyntheticpeptidecorrespondingtoaregion(197-210)fromtheC-terminusofhumanglutathioneS-transferasepi.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA.AntigenLocation197-210OtherNamesGSTP1;FAEES3;GST3;GSTclass-pi;GSTP1-1;AccessionP09211GSTP1_HUMAN;ProducedinRabbitPurityAffinitypurifiedonantigencolumnApplicationsWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanglutathioneS-transferasepihasapredictedlengthof210residuesandMWof23kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWB(Human,mouse)againsttheantigen.Cross-reactivityHuman;mouse;BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.FormLyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3ReconstitutionReconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.StorageAfterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcyclesExpiryDate12monthsafterpurchase

Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。

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