首页 > Biosensis > Biosensis/Sheep antibody to glutathione peroxidase 1 (GPx-1; 175-194): whole serum/S-072-100/100 µl

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Biosensis/Sheep antibody to glutathione peroxidase 1 (GPx-1; 175-194): whole serum/S-072-100/100 µl

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S-072-100

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DescriptionGlutathioneperoxidase1hasaroleindetoxificationofhydrogenperoxideandisoneofthemostimportantantioxidantenzymesinhumans.Itexistsasahomotetramerwhichlocalisestothecytoplasm.Itbelongstotheglutathioneperoxidasefamily.Glutathioneperoxidase1isoneoffewproteinsinhighervertebratestocontainselenocysteine,whichoccursattheactivesiteofglutathioneperoxidaseandiscodedbyUGA,thatnormallyfunctionsasatranslationterminationcodon.ThisproteinhasapolyalaninesequencepolymorphismintheN-terminalregion,whichincludesthreealleleswithfive,sixorsevenalaninerepeats.Theallelewithfivealaninerepeatsissignificantlyassociatedwithbreastcancerrisk.Twoalternativelysplicedisoformshavebeenidentified.BatchNo.SeeproductlabelUnitsize100µlAntigenAsyntheticpeptide(RRYSRRFQTIDIEPDIEALL)correspondingtotheaminoacids175-194ofhumanlutathioneperoxidase1(GPx-1)conjugatedtodiphtheriatoxinhasbeenusedastheimmunogen.OtherNamesGSHPx-1;GPx-1;Cellularglutathioneperoxidase;GPX1AccessionGPX1_HUMANProducedinSheepPurityWholeserumApplicationsIHC,WB.ThisantibodyworkssuperblyinImmunohistochemistryonfrozenorparaffinembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.Typicalworkingdilutionsforroutineimmunohistochemistryare1:2000to1:20000dependingontissueanddetectionmethod.Forwesternblottingadilutionrangeof1:10000to1:100000isrecommended.ForELISA,adilutionof1:2000to1:20000isrecommended.Thisantiserumhasextremelyhightitre,itstainshumanandratbrainmicrogliaintenselyandtosomeextentalsostainsneurons.Othertissueshavenotyetbeentested.OnWesternblottingunderreducingconditionsrecognisesa22kDproteininhumanbraintissueandSigmaglutathioneperoxidase(G-4013)isolatedfromhumanerythrocytes.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.SpecificityThisantibodyhasbeenshowntobespecificforglutathioneperoxidase1(GPx-1)protein.Cross-reactivityHumanandrat,otherspecieshavenotyetbeentested.BlastitIfyouwouldliketousethisproductinanotherspeicesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis.FormLyophilisedReconstitutionReconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.StorageAfterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability.ExpiryDate12monthsafterpurchaseReferences1.SukenagaY,etal.NucleicAcidsRes.15:7178-7178(1987).2.IshidaK,etal.NucleicAcidsRes.15:10051-10051(1987).3.MullenbachG.T,etal.NucleicAcidsRes.15:5484-5484(1987).4.ChadaS,etal.Genomics6:268-271(1990).5.MoscowJ.A,etal.J.Biol.Chem.267:5949-5958(1992).6.ForsbergL,etal.Hum.Mutat.13:294-300(1999).7.Kote-JaraiZ,etal.ProstateCancerProstaticDis.5:189-192(2002).8.HamanishiT,etal.Diabetes53:2455-2460(2004).9.IchimuraY,etal.J.Urol.172:728-732(2004).10.StrausbergR.L,etal.Proc.Natl.Acad.Sci.U.S.A.99:16899-16903(2002).

Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。