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Biosensis/Chicken polyclonal to Lamin A/C/C-1698-100/100 uL
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DescriptionTheLaminproteinsaremembersoftheintermediatefilamentproteinfamilybutarelocatedinsidethenucleusratherthaninthecytoplasm(1).Thelaminsfunctionasskeletalcomponentstightlyassociatedwiththeinnernuclearmembrane.OriginallytheproteinsofthenuclearCytoskeletonwerenamedLaminA,BandC,fromtoptobottomasvisualizedonSDS-PAGEgels.SubsequentlyitwasfoundthatLaminsAandCwerecodedforbyasinglegene(2),whiletheLaminBbandmaycontaintwoproteinsencodedbytwogenesnowcalledLaminB1andLaminB2.LaminAhasamassofabout74kDawhileLaminCis65kDa.TheLaminAproteinincludes98aminoacidsmissingfromLaminC,whileLaminChasaC-terminal6aminoacidpeptidenotpresentinLaminA.ApartfromtheseregionsLaminAandCareidenticalsothatantibodiesraisedagainsteitherproteinarelikelytocrossreactwiththeother,asisthecasewiththismonoclonal.Laminpolymerizationanddepolymerizationisregulatedbyphosphorylationbycyclindependentproteinkinase1(CDK1),thekeycomponentof"maturationpromotingfactor",thecentralregulatorofcelldivision.ActivityofthiskinaseincreasesduringcelldivisionandisresponsIBLeforthebreakdownofthenuclearlamina.MutationsintheLMNAgeneareassociatedwithseveralserioushumandiseases,includingEmery-Dreifussmusculardystrophy,familialpartiallipodystrophy,limbgirdlemusculardystrophy,dilatedcardiomyopathy,Charcot-Marie-Toothdiseasetype2B1,andHutchinson-Gilfordprogeriasyndrome.ThisfamilyofdiseasesbelongtoalargergroupwhichareoftenreferredtoasLaminopathies,thoughsomelaminopathiesareassociatedindefectsinLaminB1,B2oroneorotherofthenumerousnuclearlaminabindingproteins.AtruncatedversionoflaminA,commonlyknownasprogerin,causesHutchinson-Gilfordprogeriasyndrome,aformofprematureaging(3).BatchNo.SeeviallabelUnitsize100uLAntigenFulllengthrecombinanthumanLaminCAntibodyTypePolyclonalProducedinChickenApplicationsImmunocytochemistry(ICC)andWesternBlotting(WB).Adilutionof1:1,000-1:2,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforICC.Theoptimaldilutionshouldbedeterminedbytheenduser.SpecificityLaminAandLaminC.Theantibodyreactswitha74kDaand65kDabandbyWesternblotonHeLacellextract.IthasalsobeenusedsuccessfullyforimmunocytochemistryonHeLacellcultures.SpeciesAgainstHuman,bovine,porcine,mouseandrat.Itisexpectedthatitwillworkonothermammaltissues.FormLyophilizedfromPBS.Contains5%trehaloseand0.5%sodiumazide.AppearanceWhitepowderReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.StorageAfterreconstitutionoflyophilizedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.ExpiryDate12monthsafterpurchaseGeneralReferences1.Fisher,D.Z.,Chaudhary,N.,Blobel,G.CDNAsequencingofnuclearlaminsAandCrevealsprimaryandsecondarystructuralhomologytointermediatefilamentproteins.Proc.Nat.Acad.Sci.83:6450-6454(1986).2.McKeon,F.D.,Kirschner,M.W.,Caput,D.Homologiesinbothprimaryandsecondarystructurebetweennuclearenvelopeandintermediatefilamentproteins.Nature319:463-468(1986).3.Liu,B.andZhou,Z.LaminA/C,laminopathiesandprematureageing.Histol.Histopathol.23:747-763(2006).

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