首页 > Biosensis > Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1

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Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1

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Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescriptionThemouseVascularendothelialgrowthfactor(VEGF)KitisasandwichELISA.ThecaptureantibodyisaVEGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownVEGFconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundVEGFantibody.Abiotinylatedanti-mouseVEGFantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofVEGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseVEGFinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.Vascularendothelialgrowthfactor(VEGF)isadisulfidelinkedhomodimericproteinassociatedwithangiogenesis,vasculogenesisandendothelialcellgrowth.Atleast10isoformsofVEGFareproducedbyalternativesplicingwithvaryinglevelsofexpression.TheisoformsVEGF188,VEGF164andVEGF120arewidelyexpressedwhereastheisoformsVEGF205andVEGF144areuncommon.IsoformsVEGF164andVEGF120arefreelysecretedwhereasisoformVEGF188remainscellassociated.BatchNo.SeeproductlabelsAntigenThestandardandimmunogenusedintheELISAisrecombinantmouseVEGF-Aaminoacids27-190producedinandpurifiedfromaSF21insectcellexpressionsystem.OtherNamesVEGF-A;VascularpermeABIlityfactor;VPF;VEGFA;VEGF;VEGF-1(VEGF164);VEGF-2(VEGF120)AccessionQ00731VEGFA_MOUSE;SpecificityMouseVEGF.Thiskitrecognizesboththe164and120aminoacidresidueformsofmouseVEGF.StorageStoreat4°CSpecificReferencesHuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166.KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.Range15.6pg/ml-1,000pg/mlSensitivity< 2 pg/mlKitprotocolBEK-2110-1Plate_as_at_Nov2015protocol.pdf

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