首页 > Rockland > Rockland/Angiopoietin 1 Antibody/100-401-403/200 µL

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Rockland/Angiopoietin 1 Antibody/100-401-403/200 µL

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.detail-leftcolumn { float: left; font-weight: bold; width: 200px; } .detail-rightcolumn { float: left; width: 340px; } .detail-text-title2 { /* float: left; */ font-weight: bold; width: 200px; }Angiopoietin1AntibodyPropertiesDescriptionAnti-Angiopoietin1(RABBIT)Antibody-100-401-403HostRabbitTargetSpeciesMouseKnownCrossReactivityhuman,mouseClonalityPolyclonalApplicationELISA:1:5,000-1:25,000WesternBlot:1:500-1:2,000Immunohistochemistry:1:200-1:800OtherDilution:UserOptimizedPhysicalStateLiquid(sterilefiltered)ShippingConditionDryIceFormatAntiserumLabelUnconjugatedConcentration80mg/mLbyRefractometryBuffer0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2Preservative0.01%(w/v)SodiumAzideStABIlizerNoneGeneNameANG1RelevantLinksNCBI-46048213GeneID-11600UniProtKB-O08538Angiopoietin1AntibodyDescriptionBackgroundAntiAntiopoietin-1AntibodyrecognizesAngiopoietin-1(Ang-1)thathasimportanceinthedevelopmentoftheendotheliumbyregulatingtyrosinephosphorylationofthemembranereceptorTie-2/Tek.Ang-1bindingtoTie-2/Tekcausesphosphorylationofthereceptor.Ang-2competesforthisbinding,andthusblocksreceptorphosphorylation.Ang-1hasapotentialfibrinogen-likedomainatthecarboxylterminusandcoiled-coilregionsintheaminoterminus.Ang-1isprominentlyexpressedinthemyocardiumofatriumandventricle,mesenchymalandsmoothmusclecellssurroundingmostbloodvessels,andlung.Intheadult,Ang-1isalsoexpressedintheheartandliver.Thisantibodyissuitableforcardiovascularresearch.Synonymsrabbitanti-Angiopoietin1Antibody,rabbitanti-ANG1antibody,AGP1antibody,AGP1antibody,AGPTantibody,ANG1antibody,ANG1antibody,Angiopoietin1antibody,ANGPT1antibody,ANGPT1antibody,KIAA0003antibodyImmunogenThiswholerabbitserumwaspreparedbyrepeatedimmunizationswithasyntheticpeptideN-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Qcorrespondingtoaregion(aa21-40)neartheN-terminusofmouseangiopoietin1proteinconjugatedtoKLHusingmaleimide.Aresidueofcysteinewasaddedtotheaminoterminalendtofacilitatecoupling.ImmunogenTypePeptideStorageConditionStorevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.ApplicationNoteSuitableforwesternblotting,Immunohistochemistry(IHC)andotherantibodybasedassays. A1:500dilutionisrecommendedforwesternblotting. Thereactionofthisantiserumdirectlywithcellsupernatantsmayresultinhighbackgroundduetoreactivityofcomponentsintheserum. Thiscanbealleviatedbyfirstimmunoprecipitatingtheantibody:antigencomplexandthendetectingtheantigen.Thismethodresultsinaverycleanandstrongsignal. BothAng-1andAng-2proteinshavepredictedmolecularweightsofapproximately57kDaandappearonwesternblotsclosetotheirpredictedmolecularweights. Insomeinstancesadditionalbandsmaybeseenatapproximately75kDawhichrepresenthighlyglycosylatedformsoftheproteinthatmigrateatahigherapparentmolecularweight.Purity/SpecifityAntiopoietin1isdirectedagainstmouseangiopoietin1andshowsnoreactivitywithmouseangiopoietin2. A57kDabandisdetectedwhenassayedbyimmunoblot. Thisreagentcross-reactswithhumanangiopoietin1. Partiallycrossreactivityisnotedwithhumanangiopoietin2.DisclaimerNote-GeneralThisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。

美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。

Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。