提醒:代购产品,无质量问题不接受退换货,下单前请仔细核对信息。下单后请及时联系客服 核对商品价格,订单生效后再付款。
Rockland/AKT1 Antibody/200-301-I51/100 µg
价格:

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号:
品牌:
会员服务:
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考,请联系客服询价。
免费咨询热线
4000-520-616
产品说明
.detail-leftcolumn { float: left; font-weight: bold; width: 200px; } .detail-rightcolumn { float: left; width: 340px; } .detail-text-title2 { /* float: left; */ font-weight: bold; width: 200px; }AKT1AntibodyPropertiesDescriptionAnti-AKT1(MOUSE)MonoclonalAntibody-200-301-I51HostMouseTargetSpeciesHumanKnownCrossReactivityhumanClonalityMonoclonal14E5.A2.B2.H9IgG2akappaApplicationELISA:1:2,000-1:10,000FlowCytometry:UserOptimizedWesternBlot:1:1000Immunohistochemistry:20µg/mLOtherDilution:UserOptimizedPhysicalStateLiquid(sterilefiltered)ShippingConditionDryIceFormatIgG2aLabelUnconjugatedConcentration1.0mg/mlbyUVabsorbanceat280nmBuffer0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2Preservative0.01%(w/v)SodiumAzideStABIlizerNoneGeneNameAKT1AKT1AntibodyDescriptionBackgroundAKT1AntibodydetectsAKT1whichisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT1AntibodyisidealforinvestigatorsinvolvedinCellSignaling,NeuroscienceandSignalTransductionresearch.Synonymsmouseanti-AKT1antibody,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gammaImmunogenAnti-AKT1AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT1proteinfollowedbymonoclonaldevelopment.ImmunogenTypePeptideStorageConditionStorevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.ApplicationNoteAnti-AKT1AntibodyissuitableforELISA,immunohistochemistry,flowcytometry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT1proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.Purity/SpecifityAnti-AKT1antibodyisdirectedagainsthumanAKT1.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT1antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows85%identitytomouseand92%identitywithrat,therefore,crossreactivityisexpected.DisclaimerNote-GeneralThisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

美国Rockland是一家老牌抗体原产企业,其位于美国费城郊区的农场保有近20种实验动物,全部按美国农业部实验动物规范饲养和操作,是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产,国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品,以全面,价格合理为特色。今年,Rockland也加大了在一抗方面的投资力度,新生物分子的抗体数量每年都大幅增长,而这又是以其强大的重组蛋白表达能力为基础的。

美国Rockland公司为生命科学领域提供丰富的、高质量的抗体,包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素(Ubiquitin)等识别抗体,应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外,Rockland也生产特别齐全的二抗,包括纯化、生物素、HRP/ALP、荧光标记二抗,应用十分广泛。Rockland还对部分设施进行了GMP改造,以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图,提高其他产品的质量标准和管理水平。

Rockland还不断与时俱进,探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利,Rockland加快和科研单位合作,从深度和广度两维加速开发新产品,正在从一个OEM老厂走到前台,缩短与直接客户的距离。