Tested ApplicationsPositive IP detected inHeLa cellsPositive IHC detected inhuman gliomas tissue, human ovary tumor tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inhuman brain(ALS) tissuePositive FC detected inK-562 cellsPlanning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.Recommended dilutionApplicationDilutionImmunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WBImmunohistochemistry (IHC)IHC : 1:500-1:2500Immunofluorescence (IF)IF : 1:20-1:200Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 2 publications belowWBSee 5 publications belowIHCSee 2 publications belowIFSee 3 publications belowProduct Information60160-1-Ig targets FUS/TLS inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.Tested Reactivityhuman, mouseCited Reactivity Drosophila, humanHost / IsotypeMouse / IgG1ClassMonoclonalTypeAntibodyImmunogenFUS/TLS fusion protein Ag2150Full Namefusion (involved in t(12;16) in malignant liposarcoma)Calculated molecular weight 75 kDaObserved molecular weight 75 kDaGenBank accession numberBC026062Gene symbolFUSGene ID (NCBI)2521ConjugateUnconjugatedFormLiquidPurification MethodProtein G purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationFUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.ProtocolsProduct Specific ProtocolsWB protocol for FUS/TLS antibody 60160-1-IgDownload protocolIHC protocol for FUS/TLS antibody 60160-1-IgDownload protocolIP protocol for FUS/TLS antibody 60160-1-IgDownload protocolFC protocol for FUS/TLS antibody 60160-1-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols