Tested ApplicationsPositive WB detected inJurkat cells,A431 cellsPositive IP detected inA431 cellsPositive IHC detected inhuman prostate cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:600-1:2000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:200Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 3 publications belowWBSee 23 publications belowIHCSee 5 publications belowIFSee 3 publications belowProduct Information10048-2-Ig targets RB1 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human,mouse samples.Tested Reactivityhuman,mouseCited Reactivity human, mouseHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenRB1 fusion protein Ag0006Full Nameretinoblastoma 1Calculated molecular weight 110 kDaObserved molecular weight 110 kDaGenBank accession numberBC040540Gene symbolRB1Gene ID (NCBI)5925ConjugateUnconjugatedFormLiquidPurification MethodProtein A purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationRB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 "Lys-20" trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1"s activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.ProtocolsProduct Specific ProtocolsWB protocol for RB1 antibody 10048-2-IgDownload protocolIHC protocol for RB1 antibody 10048-2-IgDownload protocolIP protocol for RB1 antibody 10048-2-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols