Tested ApplicationsPositive WB detected infetal human brain tissue, HEK-293 cells,Neuro-2a cells,ROS1728 cells,human brain tissue,rat brain tissue,mouse brain tissue,pig brain tissuePositive IP detected inmouse brain tissuePositive IHC detected inhuman breast cancer tissue,human colon cancer tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive FC detected inHeLa cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:20000-1:50000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 1 publications belowWBSee 5 publications belowIHCSee 3 publications belowProduct Information60309-1-Ig targets pan Ras inWB, IP, IHC, FC, ELISA applications and shows reactivity with human,mouse,rat,pig samples.Tested Reactivityhuman,mouse,rat,pigCited Reactivity human, swineHost / IsotypeMouse / IgG1ClassMonoclonalTypeAntibodyImmunogenpan Ras fusion protein Ag2700Full Namev-Ki-ras2 Kirsten rat sarcoma viral oncogene homologCalculated molecular weight 188 aa, 21 kDaObserved molecular weight 21 kDaGenBank accession numberBC013572Gene symbolKRASGene ID (NCBI)3845ConjugateUnconjugatedFormLiquidPurification MethodProtein G purificationStorage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationThe 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.ProtocolsProduct Specific ProtocolsWB protocol for pan Ras antibody 60309-1-IgDownload protocolIHC protocol for pan Ras antibody 60309-1-IgDownload protocolIP protocol for pan Ras antibody 60309-1-IgDownload protocolFC protocol for pan Ras antibody 60309-1-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols