Tested ApplicationsPositive WB detected inmouse brain tissue, MCF-7 cells,Jurkat cells,rat brain tissuePositive IHC detected inhuman breast cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:1000-1:4000Immunohistochemistry (IHC)IHC : 1:100-1:400Sample-dependent, check data in validation data galleryPublished ApplicationsWBSee 1 publications belowProduct Information55103-1-AP targets CDK3 inWB, IHC, ELISA applications and shows reactivity with human,mouse samples.Tested Reactivityhuman,mouseCited Reactivity mouseHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenPeptideFull Namecyclin-dependent kinase 3Calculated molecular weight 35 kDaObserved molecular weight 40-45 kDaGenBank accession numberNM_001258Gene symbolCDK3Gene ID (NCBI)1018ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage Buffer0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Aliquoting is unnecessary for-20oC storage.Background InformationCDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.ProtocolsProduct Specific ProtocolsWB protocol for CDK3 antibody 55103-1-APDownload protocolIHC protocol for CDK3 antibody 55103-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols