Tested ApplicationsPositive WB detected inHEK-293 cells, MCF-7 cells,A431 cells,HeLa cells,HepG2 cells,C2C12 cells,C6 cellsPositive IHC detected inhuman cervical cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHeLa cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:2000Immunohistochemistry (IHC)IHC : 1:100-1:400Immunofluorescence (IF)IF : 1:20-1:200Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 2 publications belowWBSee 66 publications belowIHCSee 7 publications belowIFSee 1 publications belowCoIPSee 1 publications belowProduct Information19532-1-AP targets CDK1-Specific inWB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.Tested Reactivityhuman, mouse, ratCited Reactivity human, mouse, rat, sheepHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenPeptideFull Namecell division cycle 2, G1 to S and G2 to MCalculated molecular weight 34 kDaObserved molecular weight 34 kDaGenBank accession numberNM_001786Gene symbolCDC2Gene ID (NCBI)983ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationCDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. ProtocolsProduct Specific ProtocolsWB protocol for CDK1-Specific antibody 19532-1-APDownload protocolIHC protocol for CDK1-Specific antibody 19532-1-APDownload protocolIF protocol for CDK1-Specific antibody 19532-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols