Tested ApplicationsPositive WB detected inNIH/3T3 cells, K-562 cells,MCF-7 cells,HepG2 cellsPositive IP detected inK-562 cellsPositive IHC detected inhuman kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:2000-1:5000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WBImmunohistochemistry (IHC)IHC : 1:20-1:200Sample-dependent, check data in validation data galleryPublished ApplicationsWBSee 4 publications belowProduct InformationThe immunogen of 23762-1-AP is RSK2 Fusion Protein expressed in E. coli.Tested Reactivityhuman,mouseCited Reactivity humanHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenRSK2 fusion protein Ag20620Full Nameribosomal protein S6 kinase, 90kDa, polypeptide 3Calculated molecular weight 740 aa, 84 kDaObserved molecular weight 84 kDaGenBank accession numberBC096301Gene symbolRPS6KA3Gene ID (NCBI)6197ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationRPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.ProtocolsProduct Specific ProtocolsWB protocol for RSK2 antibody 23762-1-APDownload protocolIHC protocol for RSK2 antibody 23762-1-APDownload protocolIF protocol for RSK2 antibody 23762-1-APDownload protocolIP protocol for RSK2 antibody 23762-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols