Tested ApplicationsPositive WB detected inJurkat cells, HEK-293 cells,LNCaP cells,MCF-7 cells,mouse liver tissue,PC-3 cellsPositive IP detected inHEK-293 cellsPositive IF detected inMCF-7 cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:1000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WBImmunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 1 publications belowWBSee 10 publications belowProduct InformationThe immunogen of 27155-1-AP is Caspase 7/p20 Fusion Protein expressed in E. coli.Tested Reactivityhuman,mouseCited Reactivity human, mouse, ratHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenCaspase 7/p20 fusion protein Ag25904Full Namecaspase 7, apoptosis-related cysteine peptidaseCalculated molecular weight 303 aa, 34 kDaObserved molecular weight 35 kDaGenBank accession numberBC015799Gene symbolCASP7Gene ID (NCBI)840ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationCaspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.ProtocolsProduct Specific ProtocolsWB protocol for Caspase 7/p20 antibody 27155-1-APDownload protocolIF protocol for Caspase 7/p20 antibody 27155-1-APDownload protocolIP protocol for Caspase 7/p20 antibody 27155-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols