Tested ApplicationsPositive WB detected inHEK-293 cells, HeLa cells,mouse liver tissue,Jurkat cells,human placenta tissue,mouse brain tissue,rat brain tissuePositive IP detected inHEK-293 cellsPositive IHC detected inhuman liver cancer tissue, human lung tissue,human placenta tissue,human testis tissue,human skin tissue,human heart tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHEK-293 cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:3000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Immunofluorescence (IF)IF : 1:10-1:100Sample-dependent, check data in validation data galleryPublished ApplicationsWBSee 80 publications belowIHCSee 9 publications belowIFSee 5 publications belowIPSee 1 publications belowProduct Information10269-1-AP targets SOD1 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.Tested Reactivityhuman, mouse, ratCited Reactivity broiler chick, human, mouse, rat, swineHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenSOD1 fusion protein Ag0335Full Namesuperoxide dismutase 1, solubleCalculated molecular weight 16 kDaObserved molecular weight 16-20 kDaGenBank accession numberBC001034Gene symbolSOD1Gene ID (NCBI)6647ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationSuperoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.ProtocolsProduct Specific ProtocolsWB protocol for SOD1 antibody 10269-1-APDownload protocolIHC protocol for SOD1 antibody 10269-1-APDownload protocolIF protocol for SOD1 antibody 10269-1-APDownload protocolIP protocol for SOD1 antibody 10269-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols