Tested ApplicationsPositive WB detected inSH-SY5Y cells, HeLa cells,K-562 cells,C2C12 cells,Neuro-2a cellsPositive IHC detected inmouse brain tissue, human brain tissue,human brain (FTLD-U) tissue,human gliomas tissue,human pancreas tissue,rat brain tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHepG2 cells, HeLa cells,SH-SY5Y cellsPositive FC detected inHeLa cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:2000-1:10000Immunohistochemistry (IHC)IHC : 1:1000-1:4000Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 15 publications belowWBSee 464 publications belowIHCSee 555 publications belowIFSee 308 publications belowIPSee 32 publications belowELISASee 3 publications belowCoIPSee 4 publications belowChIPSee 4 publications belowRIPSee 2 publications belowProduct Information10782-2-AP targets TDP-43 inWB, RIP, IP, IHC, IF, IEM, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.Tested Reactivityhuman, mouse, rat, zebrafishCited Reactivity C. elegans, chicken, dog, Drosophila, fly, hamster, horse, human, monkey, mouseHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenfusion proteinFull NameTAR DNA binding proteinCalculated molecular weight 43 kDaObserved molecular weight 43 kDaGenBank accession numberBC001487Gene symbolTARDBPGene ID (NCBI)23435ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationThe TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech"s 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications.Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)ProtocolsProduct Specific ProtocolsWB protocol for TDP-43 antibody 10782-2-APDownload protocolIHC protocol for TDP-43 antibody 10782-2-APDownload protocolIF protocol for TDP-43 antibody 10782-2-APDownload protocolFC protocol for TDP-43 antibody 10782-2-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols