Tested ApplicationsPositive WB detected inSH-SY5Y cells, HeLa cellsPositive IHC detected inhuman brain tissue,human gliomas tissue,human lung cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inmouse brain tissueRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:300-1:1500Immunohistochemistry (IHC)IHC : 1:20-1:200Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsWBSee 8 publications belowIHCSee 2 publications belowIFSee 2 publications belowProduct Information10274-1-AP targets TAU inWB, IHC, IF, ELISA applications and shows reactivity with human,mouse,rat samples.Tested Reactivityhuman,mouse,ratCited Reactivity human, mouse, ratHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenTAU fusion protein Ag0354Full Namemicrotubule-associated protein tauCalculated molecular weight 37-46, 79-81 kDaObserved molecular weight 66-70 kDa, 100 kDaGenBank accession numberBC000558Gene symbolMAPTGene ID (NCBI)4137ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationTau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 70-kDa and 100-kDa bands in brain tissues.ProtocolsProduct Specific ProtocolsWB protocol for TAU antibody 10274-1-APDownload protocolIHC protocol for TAU antibody 10274-1-APDownload protocolIF protocol for TAU antibody 10274-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols