Tested ApplicationsPositive WB detected inA549 cells, HepG2 cells,MCF-7 cells,HeLa cells,HEK-293 cells,NIH/3T3 cells,C6 cellsPositive IP detected inHeLa cellsPositive IHC detected inhuman ovary tumor tissue, human cervical cancer tissue,human breast cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHeLa cellsPositive FC detected inHeLa cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:1000-1:8000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBImmunohistochemistry (IHC)IHC : 1:200-1:1000Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 6 publications belowWBSee 346 publications belowIHCSee 9 publications belowIFSee 1 publications belowIPSee 1 publications belowProduct Information10176-2-AP targets AKT inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.Tested Reactivityhuman, mouse, ratCited Reactivity A. sphaerocephala Krasch, blunt snout bream, carp, chicken, cow, fish, hamster, human, mouse, ratHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenAKT fusion protein Ag0213Full Namev-akt murine thymoma viral oncogene homolog 1Calculated molecular weight 56 kDaObserved molecular weight 56-62 kDaGenBank accession numberBC000479Gene symbolAKT1Gene ID (NCBI)207ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationThe serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.ProtocolsProduct Specific ProtocolsWB protocol for AKT antibody 10176-2-APDownload protocolIHC protocol for AKT antibody 10176-2-APDownload protocolIF protocol for AKT antibody 10176-2-APDownload protocolIP protocol for AKT antibody 10176-2-APDownload protocolFC protocol for AKT antibody 10176-2-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols