Tested ApplicationsPositive WB detected inJurkat cells, mouse spleen tissue,HeLa cells,rat brain tissue,rat liver tissuePositive IP detected inNIH/3T3 cellsPositive IHC detected inmouse brain tissue, human teeth tissue,human spleen tissue,human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inNIH/3T3 cells, mouse brain tissue,HeLa cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:500-1:2000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 2 publications belowWBSee 708 publications belowIHCSee 64 publications belowIFSee 21 publications belowELISASee 1 publications belowProduct Information19677-1-AP targets Caspase 3/p17/p19 inWB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.Tested Reactivityhuman, mouse, ratCited Reactivity Astragalus membranaceus, Bovine, chicken, cow, duck, goat, Hamster, hamsters, human, monkeyHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenPeptideFull Namecaspase 3, apoptosis-related cysteine peptidaseCalculated molecular weight 32 kDaObserved molecular weight 32-35 kDa, 17 kDa, 19 kDaGenBank accession numberNM_004346Gene symbolCASP3Gene ID (NCBI)836ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationCaspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.ProtocolsProduct Specific ProtocolsWB protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocolIHC protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocolIF protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocolIP protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols