Tested ApplicationsPositive WB detected inHeLa cells, U-937 cells,mouse spleen tissue,mouse pancreas tissue,mouse lung tissue,SH-SY5Y cells,K-562 cells,Y79 cells,mouse eye tissue,mouse kidney tissue,NIH/3T3 cellsPositive IP detected inHeLa cellsPositive IHC detected inhuman liver cancer tissue, human colon cancer tissue,human kidney tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Positive IF detected inHepG2 cells, human skin cancer tissue,NIH/3T3 cellsPositive FC detected inHEK-293 cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:2000-1:10000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WBImmunohistochemistry (IHC)IHC : 1:50-1:500Immunofluorescence (IF)IF : 1:50-1:500Sample-dependent, check data in validation data galleryPublished ApplicationsWBSee 333 publications belowIHCSee 1 publications belowIFSee 11 publications belowChIPSee 1 publications belowProduct Information12987-1-AP targets Lamin B1 inWB, IP, IHC, IF, FC, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.Tested Reactivityhuman, mouse, ratCited Reactivity canine, chicken, hamster, human, mouse, rat, swineHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenLamin B1 fusion protein Ag3631Full Namelamin B1Calculated molecular weight 66 kDaObserved molecular weight 66-70 kDaGenBank accession numberBC012295Gene symbolLMNB1Gene ID (NCBI)4001ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20oC storage.Background InformationLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.ProtocolsProduct Specific ProtocolsWB protocol for Lamin B1 antibody 12987-1-APDownload protocolIHC protocol for Lamin B1 antibody 12987-1-APDownload protocolIF protocol for Lamin B1 antibody 12987-1-APDownload protocolIP protocol for Lamin B1 antibody 12987-1-APDownload protocolFC protocol for Lamin B1 antibody 12987-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols