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ProteinTech/MEK1-Specific Polyclonal antibody/150ul/51080-1-AP

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Tested ApplicationsPositive IHC detected inhuman endometrial cancer tissue,human colon tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0Recommended dilutionApplicationDilutionImmunohistochemistry (IHC)IHC : 1:20-1:200Sample-dependent, check data in validation data galleryPublished ApplicationsWBSee 2 publications belowProduct Information51080-1-AP targets MEK1-Specific inWB, IHC, ELISA applications and shows reactivity with human samples.Tested ReactivityhumanCited Reactivity humanHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenPeptideFull Namemitogen-activated protein kinase kinase 1Calculated molecular weight 43 kDaGenBank accession numberBC139729Gene symbolMAP2K1Gene ID (NCBI)5604ConjugateUnconjugatedFormLiquidPurification MethodAntigen affinity purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Aliquoting is unnecessary for-20oC storage.Background InformationMEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. It can be phosphorylated(PMID:21144847). This antibody is specific to MEK1.ProtocolsProduct Specific ProtocolsIHC protocol for MEK1-Specific antibody 51080-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols

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