Tested ApplicationsPositive WB detected inHT-1080 cells, HeLa cells,COLO 320 cells,Raji cells,rat kidney tissue,HEK-293 cells,RAW 264.7 cells,mouse kidney tissue,rat lung tissue,PC-12 cellsPositive IP detected inRaji cellsPositive FC detected inRaji cells, HeLa cellsRecommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:5000-1:12000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WBSample-dependent, check data in validation data galleryPublished ApplicationsKD/KOSee 3 publications belowWBSee 1037 publications belowIHCSee 67 publications belowIFSee 13 publications belowIPSee 1 publications belowFCSee 1 publications belowCoIPSee 1 publications belowChIPSee 1 publications belowProduct Information50599-2-Ig targets BAX inWB, IP, IHC, FC, CoIP, chIP, ELISA applications and shows reactivity with human,mouse,rat samples.Tested Reactivityhuman,mouse,ratCited Reactivity Astragalus membranaceus, Bovine, chicken, cow, goat, guinea pig, hamster, hamsters, human, mouseHost / IsotypeRabbit / IgGClassPolyclonalTypeAntibodyImmunogenBAX fusion protein Ag0576Full NameBCL2-associated X proteinCalculated molecular weight 21 kDaObserved molecular weight 21 kDaGenBank accession numberBC014175Gene symbolBAXGene ID (NCBI)581ConjugateUnconjugatedFormLiquidPurification MethodProtein A purificationStorage BufferPBS with 0.1% sodium azide and 50% glycerol pH 7.3.Storage ConditionsStore at -20°C. Aliquoting is unnecessary for-20oC storage.Background InformationBAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1–BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL (PMIDs: 28755482, 21458670).In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion (PMID:10766872). The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C (PMID:25458844).BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human baxgene has been shown to be directly regulated by p53, and the baxpromoter contains four motifs with homology to consensus p53-binding sites (PMID:7834749). Furthermore, p53 directly interacts with BAX to promote its activation (PMID:14963330).What is the molecular weight of BAX?BAX is a 192 amino acid protein that has a predicted molecular weight of 21.1 kDa.What is the subcellular localization of BAX?In healthy mammalian cells, BAX is localized to the cytoplasm. In response to apoptotic stimuli, BAX translocates to the outer mitochondrial membrane.What is the tissue specificity of BAX?BAX is ubiquitously expressed (PMID: 25613900).What is the connection between BAX and cancer?BAX appears to play an important role in suppressing cancer development, and decreased BAX levels are associated with chemo- and radioresistance in a number of cancers including lung cancer, chronic lymphocytic leukemia (CLL), and prostate cancer. Loss-of-function mutations of BAX have also been reported in hematopoietic malignancies in humans (PMID:9531611) and in gastrointestinal cancer of the microsatellite mutator phenotype (MMP), where BAXinactivation contributes to tumor progression by providing a survival advantage (PMID:9331106).A number of anticancer drugs used clinically have been demonstrated to induce BAX activation indirectly to facilitate apoptosis of tumor cells. Recent efforts have demonstrated that BAX itself may be a promising direct target for small-molecule drug discovery for novel anticancer drugs and several direct BAX activators have been identified that may hold promise for cancer therapy, with the potential to overcome chemo- and radioresistance (PMID:25230299).ProtocolsProduct Specific ProtocolsWB protocol for BAX antibody 50599-2-IgDownload protocolIP protocol for BAX antibody 50599-2-IgDownload protocolFC protocol for BAX antibody 50599-2-IgDownload protocolStandard ProtocolsClick here to view our Standard Protocols